ArticleBiophysical journal2014
Structural determinants of water permeation through the sodium-galactose transporter vSGLT.
Article in Biophysical journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 29 citations in OpenAlex.
- Interactions of Arachidonic Acid with AAC1 and UCP1.International journal of molecular sciences · 2025Article
- Metabolic Energy is Stored in a Homeostatic Trans-Membrane Water Barochemical Gradient.The Journal of membrane biology · 2025Article
- Molecular mechanisms and computational insights into human SGLTs: advancing toward selective SGLT1 inhibition.Frontiers in molecular biosciences · 2025Review
- A Futile Cycle?: Tissue Homeostatic Trans-Membrane Water Co-Transport: Kinetics, Thermodynamics, Metabolic Consequences.bioRxiv : the preprint server for biology · 2024Article
- Collective Domain Motion Facilitates Water Transport in SGLT1.International journal of molecular sciences · 2023Article
- Cerebrospinal fluid production by the choroid plexus: a century of barrier research revisited.Fluids and barriers of the CNS · 2022Review
- Modeling of SGLT1 in Reconstituted Systems Reveals Apparent Ion-Dependencies of Glucose Uptake and Strengthens the Notion of Water-Permeable Apo States.Frontiers in physiology · 2022Article
- Paracellular Filtration Secretion Driven by Mechanical Force Contributes to Small Intestinal Fluid Dynamics.Medical sciences (Basel, Switzerland) · 2021Article
- A kinetic mechanism for enhanced selectivity of membrane transport.PLoS computational biology · 2020Article
- Mapping of Ion and Substrate Binding Sites in Human Sodium Iodide Symporter (hNIS).Journal of chemical information and modeling · 2020Article
- Computational Estimation of Microsecond to Second Atomistic Folding Times.Journal of the American Chemical Society · 2019Article
- Novel and Unexpected Functions of SGLTs.Physiology (Bethesda, Md.) · 2017Review
- Directional Fluid Transport across Organ-Blood Barriers: Physiology and Cell Biology.Cold Spring Harbor perspectives in biology · 2017Review
- Structural and functional significance of water permeation through cotransporters.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Stochastic steps in secondary active sugar transport.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- The Sodium Glucose Cotransporter SGLT1 Is an Extremely Efficient Facilitator of Passive Water Transport.The Journal of biological chemistry · 2016Article
- Membrane Protein Properties Revealed through Data-Rich Electrostatics Calculations.Structure (London, England : 1993) · 2015Article
- Computational characterization of structural dynamics underlying function in active membrane transporters.Current opinion in structural biology · 2015Review
- Article
- Metadynamics simulations reveal a Na+ independent exiting path of galactose for the inward-facing conformation of vSGLT.PLoS computational biology · 2014Article
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
Sodium-glucose transporters (SGLTs) facilitate the movement of water across the cell membrane, playing a central role in cellular homeostasis. Here, we present a detailed analysis of the mechanism of water permeation through the inward-facing state of vSGLT based on nearly 10 μs of molecular dynamics simulations. These simulations reveal the transient formation of a continuous water channel through the transporter that permits water to permeate the protein. Trajectories in which spontaneous release of galactose is observed, as well as those in which galactose remains in the binding site, show that the permeation rate, although modulated by substrate occupancy, is not tightly coupled to substrate release. Using a, to our knowledge, novel channel-detection algorithm, we identify the key residues that control water flow through the transporter and show that solvent gating is regulated by side-chain motions in a small number of residues on the extracellular face. A sequence alignment reveals the presence of two insertion sites in mammalian SGLTs that flank these outer-gate residues. We hypothesize that the absence of these sites in vSGLT may account for the high water permeability values for vSGLT determined via simulation compared to the lower experimental estimates for mammalian SGLT1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.