ArticleGenetics2014
TALENs mediate efficient and heritable mutation of endogenous genes in the marine annelid Platynereis dumerilii.
Article in Genetics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 60 citations in OpenAlex.
- Photoreceptor control ofProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Molecular profiles, sources and lineage restrictions of stem cells in an annelid regeneration model.Nature communications · 2024Article
- Dynamic microvilli sculpt bristles at nanometric scale.Nature communications · 2024Article
- Molecular circadian rhythms are robust in marine annelids lacking rhythmic behavior.PLoS biology · 2024Article
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- A Cryptochrome adopts distinct moon- and sunlight states and functions as sun- versus moonlight interpreter in monthly oscillator entrainment.Nature communications · 2022Article
- Towards an Understanding of Circatidal Clocks.Frontiers in physiology · 2022Article
- Annelid genomes: Enchytraeus crypticus, a soil model for the innate (and primed) immune system.Lab animal · 2021Article
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- TMT-Opsins differentially modulate medaka brain function in a context-dependent manner.PLoS biology · 2021Article
- Corazonin signaling integrates energy homeostasis and lunar phase to regulate aspects of growth and sexual maturation inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- Neural circuitry of a polycystin-mediated hydrodynamic startle response for predator avoidance.eLife · 2018Article
- PdumBase: a transcriptome database and research tool for Platynereis dumerilii and early development of other metazoans.BMC genomics · 2018Article
- Ciliary and rhabdomeric photoreceptor-cell circuits form a spectral depth gauge in marine zooplankton.eLife · 2018Article
- An Overview of Monthly Rhythms and Clocks.Frontiers in neurology · 2017Review
- Genome Editing in Astyanax mexicanus Using Transcription Activator-like Effector Nucleases (TALENs).Journal of visualized experiments : JoVE · 2016Article
- A serial multiplex immunogold labeling method for identifying peptidergic neurons in connectomes.eLife · 2015Article
- Cdx ParaHox genes acquired distinct developmental roles after gene duplication in vertebrate evolution.BMC biology · 2015Article
- Vasa, PL10, and Piwi gene expression during caudal regeneration of the polychaete annelid Alitta virens.Development genes and evolution · 2015Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Platynereis dumerilii is a marine polychaete and an established model system for studies of evolution and development. Platynereis is also a re-emerging model for studying the molecular basis of circalunar reproductive timing: a biological phenomenon observed in many marine species. While gene expression studies have provided new insight into patterns of gene regulation, a lack of reverse genetic tools has so far limited the depth of functional analyses in this species. To address this need, we established customized transcriptional activator-like effector nucleases (TALENs) as a tool to engineer targeted modifications in Platynereis genes. By adapting a workflow of TALEN construction protocols and mutation screening approaches for use in Platynereis, we engineered frameshift mutations in three endogenous Platynereis genes. We confirmed that such mutations are heritable, demonstrating that TALENs can be used to generate homozygous knockout lines in P. dumerilii. This is the first use of TALENs for generating genetic knockout mutations in an annelid model. These tools not only open the door for detailed in vivo functional analyses, but also can facilitate further technical development, such as targeted genome editing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.