ArticleThe Journal of cell biology1989
Transcriptional regulation of osteopontin production in rat osteoblast-like cells by parathyroid hormone.
Article in The Journal of cell biology, 1989. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 130 citations in OpenAlex.
- Serum osteopontin predicts the response to atezolizumab plus bevacizumab in patients with hepatocellular carcinoma.Journal of gastroenterology · 2023Article
- Extracellular matrix remodeling in tumor progression and immune escape: from mechanisms to treatments.Molecular cancer · 2023Review
- Comparison of Gene Expression of Different Isoforms of Osteopontin in Symptomatic Irreversible Pulpitis of Human Dental Pulp.Iranian endodontic journal · 2022Article
- Osteopontin -- a promising biomarker for cancer therapy.Journal of Cancer · 2017Review
- Biological regulation of bone quality.Current osteoporosis reports · 2014Review
- Increased osteopontin contributes to inhibition of bone mineralization in FGF23-deficient mice.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2014Article
- Article
- Changes in serum runt-related transcription factor 2 levels after a 6-month treatment with recombinant human parathyroid hormone in patients with osteoporosis.Journal of endocrinological investigation · 2012Article
- Importance of phosphorylation for osteopontin regulation of biomineralization.Calcified tissue international · 2005Article
- Fibrous dysplasia of bone in the McCune-Albright syndrome: abnormalities in bone formation.The American journal of pathology · 1997Article
- Microsomal casein kinase II in endoplasmic reticulum- and Golgi apparatus-rich fractions of ROS 17/2.8 osteoblast-like cells: an enzyme that modifies osteopontin.Calcified tissue international · 1995Article
- Macrophages express osteopontin during repair of myocardial necrosis.The American journal of pathology · 1994Article
- Cloning and characterization of the human osteopontin gene and its promoter.The Biochemical journal · 1994Article
- Age- and gender-related changes in the distribution of osteocalcin in the extracellular matrix of normal male and female bone. Possible involvement of osteocalcin in bone remodeling.The Journal of clinical investigation · 1994Article
- Inhibition of calcium oxalate crystal growth in vitro by uropontin: another member of the aspartic acid-rich protein superfamily.Proceedings of the National Academy of Sciences of the United States of America · 1992Article
- Physiology of bone: mineral compartment proteins as candidates for environmental perturbation by lead.Environmental health perspectives · 1991Review
- Identification of a DNA sequence responsible for binding of the 1,25-dihydroxyvitamin D3 receptor and 1,25-dihydroxyvitamin D3 enhancement of mouse secreted phosphoprotein 1 (SPP-1 or osteopontin) gene expression.Proceedings of the National Academy of Sciences of the United States of America · 1990Article
- Fibroblast growth factor enhances type beta 1 transforming growth factor gene expression in osteoblast-like cells.The Journal of cell biology · 1989Article
- Ultrastructural localization of osteopontin immunoreactivity in phagolysosomes and secretory granules of cells in human intestine.The Histochemical journalArticle
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Osteopontin (OP) or bone sialoprotein is a recently characterized extracellular matrix protein which is abundant in bone and is produced by osteoblasts. Parathyroid hormone (PTH) is a potent calcitropic hormone which regulates osteoblastic function including the synthesis of extracellular matrix proteins. This study examines the effect of human PTH (hPTH-[1-34]) on the expression of this novel protein in rat osteoblast-like cells. hPTH(1-34) significantly decreased the amount of OP in culture media of the rat osteoblastic osteosarcoma cell line, ROS 17/2.8, detected by Western immunoblot analysis. hPTH(1-34) also suppressed the steady-state level of OP mRNA two- to threefold with an ED50 of approximately 3 X 10(-10) M. This inhibition was detectable at 24 h, reached its nadir at 48 h, and lasted at least up to 96 h. The hPTH(1-34) effects were mimicked by isobutylmethylxanthine, cholera toxin, 8-bromo-cAMP, forskolin, and isoproterenol. hPTH(1-34) suppressed by two- to threefold the rate of OP gene transcription, estimated by nuclear run-on assays. The suppression of OP mRNA levels by hPTH(1-34) was also seen when basal levels were increased by transforming growth factor type beta, or 1,25-dihydroxyvitamin D3, or were decreased by dexamethasone. A similar decrease in the steady-state level of OP mRNA by hPTH(1-34) was also observed in primary cultures of osteoblast-enriched cells from fetal rat calvaria. These findings indicate that hPTH(1-34) suppresses the production of the novel extracellular matrix protein, OP, in osteoblasts at least in part through transcriptional control.
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