Evidence map›Paper›PMID 24651823›Full record

ArticleScientific reports2014

Structure and permeability of ion-channels by integrated AFM and waveguide TIRF microscopy.

Srinivasan Ramachandran, Fernando Teran Arce, Nirav R Patel, Arjan P Quist, Daniel A Cohen, Ratnesh Lal

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Srinivasan Ramachandran1] Department of Bioengineering; Department of Mechanical & Aerospace Engineering, University of California San Diego, La Jolla, CA 92093 [2].
Fernando Teran Arce1] Department of Bioengineering; Department of Mechanical & Aerospace Engineering, University of California San Diego, La Jolla, CA 92093 [2].
Nirav R PatelDepartment of Bioengineering; Department of Mechanical & Aerospace Engineering, University of California San Diego, La Jolla, CA 92093.
Arjan P QuistRC Nano Corporation, 2210 Midwest Road, Oak Brook, IL 60523, USA. Current Address: Innovation and New Ventures Office, Northwestern University, 1800 Sherman Ave., Evanston IL 60201.
Daniel A CohenDepartment of Materials, University of California Santa Barbara, CA 93106.
Ratnesh LalDepartment of Bioengineering; Department of Mechanical & Aerospace Engineering, University of California San Diego, La Jolla, CA 92093.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Membrane ion channels regulate key cellular functions and their activity is dependent on their 3D structure. Atomic force microscopy (AFM) images 3D structure of membrane channels placed on a solid substrate. Solid substrate prevents molecular transport through ion channels thus hindering any direct structure-function relationship analysis. Here we designed a ~70 nm nanopore to suspend a membrane, allowing fluidic access to both sides. We used these nanopores with AFM and total internal reflection fluorescence microscopy (TIRFM) for high resolution imaging and molecular transport measurement. Significantly, membranes over the nanopore were stable for repeated AFM imaging. We studied structure-activity relationship of gap junction hemichannels reconstituted in lipid bilayers. Individual hemichannels in the membrane overlying the nanopore were resolved and transport of hemichannel-permeant LY dye was visualized when the hemichannel was opened by lowering calcium in the medium. This integrated technique will allow direct structure-permeability relationship of many ion channels and receptors.

Indexed as

AnimalsBiological TransportCalciumConnexin 43FibroblastsFluorescent DyesGap JunctionsIsoquinolinesLipid BilayersMicroscopy, Atomic ForceMicroscopy, FluorescencePermeabilityPorosityRatsStructure-Activity RelationshipCalciumConnexin 43Fluorescent DyesIsoquinolinesLipid Bilayerslucifer yellow

Identifiers

PMID24651823
PMCPMC3961736

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.