ArticlePloS one2014
Direct observation of unstained biological specimens in water by the frequency transmission electric-field method using SEM.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 39 citations in OpenAlex.
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- Observation of biological and emulsion samples by newly developed three-dimensional impedance scanning electron microscopy.Computational and structural biotechnology journal · 2024Article
- Quantitative evaluation of particle-binder interactions in ceramic slurries via differential centrifugal sedimentation.Scientific reports · 2024Article
- Direct observation of the effects of chemical fixation in MNT-1 cells: A SE-ADM and Raman study.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Structural analysis of melanosomes in living mammalian cells using scanning electron-assisted dielectric microscopy with deep neural network.Computational and structural biotechnology journal · 2023Article
- Raman scattering enhancement of dielectric microspheres on silicon nitride film.Scientific reports · 2022Article
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- Nanoscale observation of PM2.5 incorporated into mammalian cells using scanning electron-assisted dielectric microscope.Scientific reports · 2021Article
- Fluorescence Enhancement Effect of TiOInternational journal of molecular sciences · 2019Article
- Osteoblastic lysosome plays a central role in mineralization.Science advances · 2019Article
- Direct observation of unstained biological samples in water using newly developed impedance scanning electron microscopy.PloS one · 2019Article
- Observation of wet specimens sensitive to evaporation using scanning electron microscopy.Microscopy (Oxford, England) · 2018Article
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- High-resolution imaging of living mammalian cells bound by nanobeads-connected antibodies in a medium using scanning electron-assisted dielectric microscopy.Scientific reports · 2017Article
- Nanoscale imaging of untreated mammalian cells in a medium with low radiation damage using scanning electron-assisted dielectric microscopy.Scientific reports · 2016Article
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Authors and funding
1 author at 1 institution in 1 country.
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Abstract
Scanning electron microscopy (SEM) is a powerful tool for the direct visualization of biological specimens at nanometre-scale resolution. However, images of unstained specimens in water using an atmospheric holder exhibit very poor contrast and heavy radiation damage. Here, we present a new form of microscopy, the frequency transmission electric-field (FTE) method using SEM, that offers low radiation damage and high-contrast observation of unstained biological samples in water. The wet biological specimens are enclosed in two silicon nitride (SiN) films. The metal-coated SiN film is irradiated using a focused modulation electron beam (EB) at a low-accelerating voltage. A measurement terminal under the sample holder detects the electric-field frequency signal, which contains structural information relating to the biological specimens. Our results in very little radiation damage to the sample, and the observation image is similar to the transmission image, depending on the sample volume. Our developed method can easily be utilized for the observation of various biological specimens in water.
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