Evidence map›Paper›PMID 24651155›Full record

ArticlePLoS pathogens2014

Production, fate and pathogenicity of plasma microparticles in murine cerebral malaria.

Fatima El-Assaad, Julie Wheway, Nicholas H Hunt, Georges E R Grau, Valery Combes

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PLoS pathogens, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 47 papers.

0numbers the graph read from it
0cells of the map it votes in
47citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

47 citing papers in PubMed.

  1. Review
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  11. Article
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  15. Article
  16. Review
  17. Erythropoiesis and Malaria, a Multifaceted Interplay.International journal of molecular sciences · 2022
    Review
  18. Brain endothelial STING1 activation byProceedings of the National Academy of Sciences of the United States of America · 2022
    Article
  19. Article
  20. Autophagy Pathways in the Genesis ofLife (Basel, Switzerland) · 2022
    Article
4 · The record

Corrections and comments

  • Erratum issued
5 · Who and what money

Authors and funding

5 authors.

Fatima El-AssaadVascular Immunology Unit, Department of Pathology, Sydney Medical School, The University of Sydney, Sydney, Australia.
Julie WhewayVascular Immunology Unit, Department of Pathology, Sydney Medical School, The University of Sydney, Sydney, Australia.
Nicholas H HuntMolecular Immunopathology Unit, Department of Pathology, Sydney Medical School, The University of Sydney, Sydney, Australia.
Georges E R GrauVascular Immunology Unit, Department of Pathology, Sydney Medical School, The University of Sydney, Sydney, Australia.
Valery CombesVascular Immunology Unit, Department of Pathology, Sydney Medical School, The University of Sydney, Sydney, Australia.

Funding

Microparticles, a new player in cerebral malaria pathogenesisR01NS079873 · NINDS · LA JOLLA INFECTIOUS DISEASE INSTITUTE · PI COMBES, VALERY MADELEINE · 2012 to 2015
$1.3M
NINDS NIH HHS 1R01NS079873-01NINDS NIH HHS R01 NS079873
6 · The paper itself

Abstract

In patients with cerebral malaria (CM), higher levels of cell-specific microparticles (MP) correlate with the presence of neurological symptoms. MP are submicron plasma membrane-derived vesicles that express antigens of their cell of origin and phosphatidylserine (PS) on their surface, facilitating their role in coagulation, inflammation and cell adhesion. In this study, the in vivo production, fate and pathogenicity of cell-specific MP during Plasmodium berghei infection of mice were evaluated. Using annexin V, a PS ligand, and flow cytometry, analysis of platelet-free plasma from infected mice with cerebral involvement showed a peak of MP levels at the time of the neurological onset. Phenotypic analyses showed that MP from infected mice were predominantly of platelet, endothelial and erythrocytic origins. To determine the in vivo fate of MP, we adoptively transferred fluorescently labelled MP from mice with CM into healthy or infected recipient mice. MP were quickly cleared following intravenous injection, but microscopic examination revealed arrested MP lining the endothelium of brain vessels of infected, but not healthy, recipient mice. To determine the pathogenicity of MP, we transferred MP from activated endothelial cells into healthy recipient mice and this induced CM-like brain and lung pathology. This study supports a pathogenic role for MP in the aggravation of the neurological lesion and suggests a causal relationship between MP and the development of CM.

Indexed as

Adoptive TransferAnimalsCell-Derived MicroparticlesDisease Models, AnimalFemaleFlow CytometryFluorescent Antibody TechniqueMalaria, CerebralMiceMice, Inbred CBAPlasmodium bergheiVirulence

Identifiers

PMID24651155
PMCPMC3961352

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.