ArticlePloS one2014
Selection of suitable reference genes for qPCR normalization under abiotic stresses in Oenanthe javanica (BI.) DC.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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36 citing papers in PubMed.
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- Ethylene response factors as potential regulators of cell wall formation in celery (Apium graveolens L.) collenchyma: phylogeny and gene expression analysis.Protoplasma · 2025Article
- Screening and validation of optimal real-time PCR reference genes for Abelmoschus Manihot.Scientific reports · 2025Article
- Evaluation of Suitable Reference Genes for Quantitative Real-Time PCR in Various Tissues ofGenes · 2024Article
- Comprehensive in silico analysis of the underutilized crop tef (Eragrostis tef (Zucc.) Trotter) genome reveals drought tolerance signatures.BMC plant biology · 2023Article
- Selection of suitable reference genes for qPCR normalization in different developmental stages ofFrontiers in plant science · 2023Article
- Systematic identification of reference genes for qRT-PCR ofFrontiers in plant science · 2023Article
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- Systematic screening and validation of reliable reference genes for qRT-PCR analysis in Okra (Abelmoschus esculentus L.).Scientific reports · 2022Article
- Integrative Analysis of Long- and Short-Read Transcriptomes Identify the Regulation of Terpenoids Biosynthesis Under Shading Cultivation inFrontiers in genetics · 2022Article
- Integrative genome, transcriptome, microRNA, and degradome analysis of water dropwort (Oenanthe javanica) in response to water stress.Horticulture research · 2021Article
- Expression Analysis of XTH in Stem Swelling of Stem Mustard and Selection of Reference Genes.Genes · 2020Article
- Validation of suitable reference genes for quantitative gene expression analysis in Tripterygium wilfordii.Molecular biology reports · 2019Article
- Selection of reference genes for flowering pathway analysis in the masting plants, Celmisia lyallii and Chionochloa pallens, under variable environmental conditions.Scientific reports · 2019Article
- Identification and validation of reference genes for real-time quantitative RT-PCR analysis in jute.BMC molecular biology · 2019Article
- Article
- A Review ofEvidence-based complementary and alternative medicine : eCAM · 2019Review
- Selection of reliable reference genes for quantitative RT-PCR in garlic under salt stress.PeerJ · 2019Article
- An R2R3-MYB transcription factor, OjMYB1, functions in anthocyanin biosynthesis in Oenanthe javanica.Planta · 2018Article
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6 authors.
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Abstract
Accurate normalization of gene expression data is an absolute prerequisite to obtain reliable results in qPCR analysis. Oenanthe javanica, an aquatic perennial herb, belongs to the Oenanthe genus in Apiaceae family, with known medicinal properties. In the current study, O. javanica was subjected to hormone stimuli (gibberellin, salicylic acid, methyl jasmonate, and abscisic acid) and abiotic stresses (heat, cold, salt, and drought), and the expression of nine candidate reference genes (eIF-4α, ACT7, TIP41, GAPDH, SAND, EF-1α, PP2A, TBP, and TUB) was evaluated. Stability of the genes was assessed using geNorm, NormFinder and BestKeeper. All the genes presented distinct expression profiles under the experimental conditions analyzed. Under abiotic stress conditions, ACT7 and PP2A genes displayed the maximum stability; PP2A and SAND were the most stable genes under hormone stimuli. Even though PP2A gene was most stable across all the samples, individual analysis revealed changes in expression profile. To further validate the suitability of the reference genes identified in this study, the expression level of M6PR gene under salt treatment was studied. Based on our data, we propose that it is essential to normalize the target gene expression with specific reference genes under different experimental conditions for most accurate results. To our knowledge, this is the first systematic analysis for reference genes under abiotic stress and hormone stimuli conditions in O. javanica. This will be beneficial for future studies on O. javanica and other plants in Apiaceae family at molecular level.
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