ArticleAnalytical chemistry2014
Parallel affinity-based isolation of leukocyte subsets using microfluidics: application for stroke diagnosis.
Article in Analytical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 30 citations in OpenAlex.
- Microfluidics for Blood Disorders and Hematological Disease Monitoring and Modeling.International journal of molecular sciences · 2026Review
- Plasma lipidomic profiling reveals six candidate biomarkers for the prediction of incident stroke in patients with hypertension.Metabolomics : Official journal of the Metabolomic Society · 2024Article
- Application of microfluidic chips in anticancer drug screening.Bosnian journal of basic medical sciences · 2022Review
- Liquid biopsy markers for stroke diagnosis.Expert review of molecular diagnostics · 2020Review
- Visible photorelease of liquid biopsy markers following microfluidic affinity-enrichment.Chemical communications (Cambridge, England) · 2020Article
- Translating microfluidics: Cell separation technologies and their barriers to commercialization.Cytometry. Part B, Clinical cytometry · 2017Review
- Discrete microfluidics for the isolation of circulating tumor cell subpopulations targeting fibroblast activation protein alpha and epithelial cell adhesion molecule.NPJ precision oncology · 2017Article
- Characterization of activated cyclic olefin copolymer: effects of ethylene/norbornene content on the physiochemical properties.The Analyst · 2016Article
- Microfluidics for the detection of minimal residual disease in acute myeloid leukemia patients using circulating leukemic cells selected from blood.The Analyst · 2016Article
- Microfluidic Organ/Body-on-a-Chip Devices at the Convergence of Biology and Microengineering.Sensors (Basel, Switzerland) · 2015Review
- Microfluidic cell sorting: a review of the advances in the separation of cells from debulking to rare cell isolation.Lab on a chip · 2015Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
We report the design and performance of a polymer microfluidic device that can affinity select multiple types of biological cells simultaneously with sufficient recovery and purity to allow for the expression profiling of mRNA isolated from these cells. The microfluidic device consisted of four independent selection beds with curvilinear channels that were 25 μm wide and 80 μm deep and were modified with antibodies targeting antigens specifically expressed by two different cell types. Bifurcated and Z-configured device geometries were evaluated for cell selection. As an example of the performance of these devices, CD4+ T-cells and neutrophils were selected from whole blood as these cells are known to express genes found in stroke-related expression profiles that can be used for the diagnosis of this disease. CD4+ T-cells and neutrophils were simultaneously isolated with purities >90% using affinity-based capture in cyclic olefin copolymer (COC) devices with a processing time of ∼3 min. In addition, sufficient quantities of the cells could be recovered from a 50 μL whole blood input to allow for reverse transcription-polymerase chain reaction (RT-PCR) following cell lysis. The expression of genes from isolated T-cells and neutrophils, such as S100A9, TCRB, and FPR1, was evaluated using RT-PCR. The modification and isolation procedures demonstrated here can also be used to analyze other cell types as well where multiple subsets must be interrogated.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.