ArticleBMC oral health2014
Analysis of human alveolar osteoblast behavior on a nano-hydroxyapatite substrate: an in vitro study.
Article in BMC oral health, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Comparing Nanohydroxyapatite Graft and Other Bone Grafts in the Repair of Periodontal Infrabony Lesions: A Systematic Review and Meta-Analysis.International journal of molecular sciences · 2021Pooled it
- Effects of platelet-rich fibrin combined with hydroxyapatite in the treatment of patients with periodontal intrabony defects: a meta-analysis.Clinics (Sao Paulo, Brazil) · 2026Review
- Article
- The comparison of physicochemical and bioactivity properties of different nanoparticles modified calcium silicate-based cement.BMC oral health · 2025Article
- Advancements in osteoblast sourcing, isolation, and characterization for dental tissue regeneration: a review.Biomedical engineering online · 2025Review
- Periapical healing in endodontic retreatment: A comparative study of nano-hydroxyapatite with and without PRF.Bioinformation · 2025Article
- Effects of Photobiomodulation Using Low-Power Diode Laser Therapy and Nano-bone on Mandibular Bone Regeneration in Rats.Journal of lasers in medical sciences · 2024Article
- Synthesis and Characterization of Nano-Hydroxyapatite Obtained from Eggshell via the Hydrothermal Process and the Precipitation Method.Molecules (Basel, Switzerland) · 2023Article
- Evaluation of safety and efficacy of the bone marrow mesenchymal stem cell and gelatin-nano-hydroxyapatite combination in canine femoral defect repair.Frontiers in veterinary science · 2023Article
- Article
- Correction of large jawbone defect in the mouse using immature osteoblast-like cells and a 3D polylactic acid scaffold.PNAS nexus · 2022Article
- Stimulation of Metabolic Activity and Cell Differentiation in Osteoblastic and Human Mesenchymal Stem Cells by a Nanohydroxyapatite Paste Bone Graft Substitute.Materials (Basel, Switzerland) · 2022Article
- Article
- Expression of Vascular Endothelial Growth Factor Using Platelet Rich Fibrin (PRF) and Nanohydroxyapatite (nano-HA) in Treatment of Periodontal Intra-Bony Defects - A Randomized Controlled Trial.Saudi journal of biological sciences · 2021Article
- Synthetic Biodegradable Aliphatic Polyester Nanocomposites Reinforced with Nanohydroxyapatite and/or Graphene Oxide for Bone Tissue Engineering Applications.Nanomaterials (Basel, Switzerland) · 2019Review
- Nano-Hydroxyapatite Coating Promotes Porous Calcium Phosphate Ceramic-Induced Osteogenesis Via BMP/Smad Signaling Pathway.International journal of nanomedicine · 2019Article
- A Molecular View of Pathological Microcalcification in Breast Cancer.Journal of mammary gland biology and neoplasia · 2016Review
- Safety of bioabsorbable implants in vitro.BMC surgery · 2015Article
- Regenerative nanomedicine: current perspectives and future directions.International journal of nanomedicine · 2014Review
- Clinical evaluation of the regenerative potential of EMD and NanoHA in periodontal infrabony defects: a 2-year follow-up.BioMed research international · 2014Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNano-hydroxyapatite (nHA) is a potential ideal biomaterial for bone regeneration. However, studies have yet to characterize the behavior of human osteoblasts derived from alveolar bone on nHA. Thus, the aim of the present study was to evaluate the influence of nHA on the adhesion, proliferation and differentiation of these alveolar bone-derived cells.
methodsPrimary human alveolar osteoblasts were collected from the alveolar ridge of a male periodontal patient during osseous resective surgery and grown on culture plates coated with either polylysine or polylysine with nano-hydroxyapatite (POL/nHA) composite. The cells were grown and observed for 14 days, and then assessed for potential modifications to osteoblasts homeostasis as evaluated by quantitative reverse transcriptase-polymerase chain reaction (real time RT-PCR), scanning electron microscopy and atomic force microscopy.
resultsReal time PCR revealed a significant increase in the expression of the selected markers of osteoblast differentiation (bone morphogenetic protein (BMP)-2,-5,-7, ALP, COLL-1A2, OC, ON) in cells grown on the POL/nHA substrate. In addition, as compared with the POL surface, cells grown on the POL/nHA substrate demonstrated better osteoconductive properties, as demonstrated by the increase in adhesion and spreading, likely as a result of the increased surface roughness of the composite.
conclusionsThe increased expression of BMPs and osteoinductive biomarkers suggest that nano-hydroxyapatite may stimulate the proliferation and differentiation of local alveolar osteoblasts and thus encourage bone regeneration at sites of alveolar bone regeneration.
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