Evidence map›Paper›PMID 24649403›Full record

ArticleFEBS open bio2014

Analysis of essential pathways for self-renewal in common marmoset embryonic stem cells.

Takenobu Nii, Tomotoshi Marumoto, Hirotaka Kawano, Saori Yamaguchi, Jiyuan Liao, Michiyo Okada, Erika Sasaki, Yoshie Miura, Kenzaburo Tani

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FEBS open bio, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
0.6field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed, 13 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Takenobu NiiDivision of Molecular and Clinical Genetics, Medical Institute of Bioregulation, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Tomotoshi MarumotoDivision of Molecular and Clinical Genetics, Medical Institute of Bioregulation, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan ; Department of Advanced Molecular and Cell Therapy, Kyushu University Hospital, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Hirotaka KawanoDivision of Molecular and Clinical Genetics, Medical Institute of Bioregulation, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Saori YamaguchiDivision of Molecular and Clinical Genetics, Medical Institute of Bioregulation, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Jiyuan LiaoDivision of Molecular and Clinical Genetics, Medical Institute of Bioregulation, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Michiyo OkadaDivision of Molecular and Clinical Genetics, Medical Institute of Bioregulation, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Erika SasakiCentral Institute for Experimental Animals, Kawasaki, Kanagawa 216-0001, Japan ; Keio Advanced Research Center, Keio University School of Medicine, Tokyo 160-8582, Japan.
Yoshie MiuraDivision of Molecular and Clinical Genetics, Medical Institute of Bioregulation, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Kenzaburo TaniDivision of Molecular and Clinical Genetics, Medical Institute of Bioregulation, Kyushu University, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan ; Department of Advanced Molecular and Cell Therapy, Kyushu University Hospital, 3-1-1, Maidashi, Higashi-ku, Fukuoka 812-8582, Japan.
Kyushu University · JPCentral Institute for Experimental Animals · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Common marmoset (CM) is widely recognized as a useful non-human primate for disease modeling and preclinical studies. Thus, embryonic stem cells (ESCs) derived from CM have potential as an appropriate cell source to test human regenerative medicine using human ESCs. CM ESCs have been established by us and other groups, and can be cultured in vitro. However, the growth factors and downstream pathways for self-renewal of CM ESCs are largely unknown. In this study, we found that basic fibroblast growth factor (bFGF) rather than leukemia inhibitory factor (LIF) promoted CM ESC self-renewal via the activation of phosphatidylinositol-3-kinase (PI3K)-protein kinase B (AKT) pathway on mouse embryonic fibroblast (MEF) feeders. Moreover, bFGF and transforming growth factor β (TGFβ) signaling pathways cooperatively maintained the undifferentiated state of CM ESCs under feeder-free condition. Our findings may improve the culture techniques of CM ESCs and facilitate their use as a preclinical experimental resource for human regenerative medicine.

Indexed as

AKT, protein kinase BbFGFbFGF, basic fibroblast growth factorCM, common marmosetCommon marmosetEB, embryoid bodyEmbryonic stem cellsEpiSCs, epiblast stem cellsERK, extracellular signal-regulated kinaseESCs, embryonic stem cellsFCM, flow cytometryiPSCs, induced pluripotent stem cellsJAK, janus kinaseKSR, knockout serum replacementLIF, leukemia inhibitory factorMEFs, mouse embryonic fibroblastsMEK, mitogen-activated protein/extracellular signal-regulated kinase kinasePI3K, phosphatidylinositol-3-kinaseRT-PCR, reverse transcription-polymerase chain reactionSelf-renewalSMAD2/3, mothers against decapentaplegic homolog 2/3STAT3, signal transducer and activator of transcription 3TGFβTGFβ, transforming growth factor β

Identifiers

PMID24649403
PMCPMC3958738
OpenAlexW1995907690

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.