ArticleJournal of clinical microbiology2014
Multiplex real-time PCR assay for rapid detection of methicillin-resistant staphylococci directly from positive blood cultures.
Article in Journal of clinical microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 63 citations in OpenAlex.
- Clinical Utility of Molecular Tests for Guiding Therapeutic Decisions in Bloodstream Staphylococcal Infections: A Meta-Analysis.Frontiers in pediatrics · 2021Pooled it
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- Quantification of Bacterial and Drug-Resistant DNA Using dPCR in a Pediatric Patient with CVC-Related Bloodstream Infection.Infectious disease reports · 2025Article
- Genomic characterization ofFrontiers in microbiology · 2025Article
- Multiplex Digital PCR-Based Development and discussion of the Detection of Genetic Association BetweenInfection and drug resistance · 2024Article
- Rapid and sensitive detection of methicillin-resistantFrontiers in microbiology · 2024Article
- Diabetes mellitus promotes the nasal colonization of high virulentEmerging microbes & infections · 2023Article
- Detection of respiratory pathogenic bacterial nucleic acid detection by Loop-mediated Isothermal Amplification in patients with bacterial pulmonary infections.Practical laboratory medicine · 2023Article
- Prevalence and Characteristics of InvasiveMicroorganisms · 2023Article
- Staphylococcus aureus causes aberrant epidermal lipid composition and skin barrier dysfunction.Allergy · 2023Article
- Whole genome sequence-based analysis ofFrontiers in microbiology · 2023Article
- Phenotypic and genotypic characterization of erythromycin-resistantVeterinary world · 2023Article
- Oral Microbiota Profile in Patients with Anti-Neutrophil Cytoplasmic Antibody-Associated Vasculitis.Microorganisms · 2022Article
- Detection of Methicillin-ResistantAntibiotics (Basel, Switzerland) · 2022Article
- Development and Clinical Application of a Recombinase Polymerase Amplification-Lateral Flow Strip Assay for Detection of Carbapenem-ResistantFrontiers in cellular and infection microbiology · 2022Article
- Bacteria Associated with Healthcare-Associated Infections on Environmental Samples Obtained from Two Fire Departments.International journal of environmental research and public health · 2021Article
- Molecular Characterization ofPathogens (Basel, Switzerland) · 2021Article
- Development of a multiplex real-time PCR assay for the simultaneous detection of four bacterial pathogens causing pneumonia.PloS one · 2021Article
- Methicillin-Resistant Coagulase-NegativeThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2021Article
- Rational Design of a User-Friendly Aptamer/Peptide-Based Device for the Detection ofSensors (Basel, Switzerland) · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) is the most prevalent cause of bloodstream infections (BSIs) and is recognized as a major nosocomial pathogen. This study aimed to evaluate a newly designed multiplex real-time PCR assay capable of the simultaneous detection of mecA, S. aureus, and coagulase-negative staphylococci (CoNS) in blood culture specimens. The Real-MRSA and Real-MRCoNS multiplex real-time PCR assays (M&D, Republic of Korea) use the TaqMan probes 16S rRNA for Staphylococcus spp., the nuc gene for S. aureus, and the mecA gene for methicillin resistance. The detection limit of the multiplex real-time PCR assay was 10(3) CFU/ml per PCR for each gene target. The multiplex real-time PCR assay was evaluated using 118 clinical isolates from various specimen types and a total of 350 positive blood cultures from a continuous monitoring blood culture system. The results obtained with the multiplex real-time PCR assay for the three targets were in agreement with those of conventional identification and susceptibility testing methods except for one organism. Of 350 positive bottle cultures, the sensitivities of the multiplex real-time PCR kit were 100% (166/166 cultures), 97.2% (35/36 cultures), and 99.2% (117/118 cultures) for the 16S rRNA, nuc, and mecA genes, respectively, and the specificities for all three targets were 100%. The Real-MRSA and Real-MRCoNS multiplex real-time PCR assays are very useful for the rapid accurate diagnosis of staphylococcal BSIs. In addition, the Real-MRSA and Real-MRCoNS multiplex real-time PCR assays could have an important impact on the choice of appropriate antimicrobial therapy, based on detection of the mecA gene.
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