ArticleJournal of clinical microbiology2014
Rapid genome detection of Schmallenberg virus and bovine viral diarrhea virus by use of isothermal amplification methods and high-speed real-time reverse transcriptase PCR.
Article in Journal of clinical microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed.
- OM-sqPCR enables one-step multiplex quantification of circulating small-RNA subclasses for colorectal cancer classification.iScience · 2026Article
- Epidemiological surveillance of BVDV, Pasteurella multocida, and Brucella spp. infections in cattle from Guizhou, China using a newly established triplex TaqMan qPCR assay.Veterinary research communications · 2026Article
- Shining a light on viral detection: a comparative study of electrochemical and electrochemiluminescence techniques for human cytomegalovirus.Analytical and bioanalytical chemistry · 2026Article
- Article
- Development of isothermal nucleic acid amplification technologies for rapid detection of Porcine Enterovirus-G.PloS one · 2025Article
- Schmallenberg virus epidemiology and regional control strategies: diagnostics, vaccines, and vector management.Frontiers in cellular and infection microbiology · 2025Review
- Advances in Virus Detection Techniques Based on Recombinant Polymerase Amplification.Molecules (Basel, Switzerland) · 2024Review
- ƩS COVID-19 is a rapid high throughput and sensitive one-step quadruplex real-time RT-PCR assay.Scientific reports · 2024Article
- Refining the Schistosoma haematobium recombinase polymerase amplification (Sh-RPA) assay: moving towards point-of-care use in endemic settings.Parasites & vectors · 2024Article
- Article
- Diagnosis of bovine viral diarrhea virus: an overview of currently available methods.Frontiers in microbiology · 2024Review
- Diagnosis of Ruminant Viral Diseases with Loop-Mediated Isothermal Amplification.Molecular biotechnology · 2023Review
- CRISPR-based assays for rapid detection of SARS-CoV-2.Methods (San Diego, Calif.) · 2022Review
- Viral Eco-Genomic Tools: Development and Implementation for Aquatic Biomonitoring.International journal of environmental research and public health · 2022Review
- An automated nucleic acid detection platform using digital microfluidics with an optimized Cas12a system.Science China. Chemistry · 2022Article
- Broad detection and quick differentiation of bovine viral diarrhea viruses 1 and 2 by a reverse transcription loop-mediated isothermal amplification test.The Journal of veterinary medical science · 2021Article
- Development and comparison of cross-linking and non-crosslinking probe-gold nanoparticle hybridization assays for direct detection of unamplified bovine viral diarrhea virus-RNA.BMC biotechnology · 2021Article
- Recombinase polymerase amplification assay combined with a dipstick-readout for rapid detection of Mycoplasma ovipneumoniae infections.PloS one · 2021Article
- Rapid and simple detection ofPhytopathology research · 2021Article
- Evaluation and improvement of isothermal amplification methods for point-of-need plant disease diagnostics.PloS one · 2020Article
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Over the past few years, there has been an increasing demand for rapid and simple diagnostic tools that can be applied outside centralized laboratories by using transportable devices. In veterinary medicine, such mobile test systems would circumvent barriers associated with the transportation of samples and significantly reduce the time to diagnose important infectious animal diseases. Among a wide range of available technologies, high-speed real-time reverse transcriptase quantitative PCR (RT-qPCR) and the two isothermal amplification techniques loop-mediated isothermal amplification (LAMP) and recombinase polymerase amplification (RPA) represent three promising candidates for integration into mobile pen-side tests. The aim of this study was to investigate the performance of these amplification strategies and to evaluate their suitability for field application. In order to enable a valid comparison, novel pathogen-specific assays have been developed for the detection of Schmallenberg virus and bovine viral diarrhea virus. The newly developed assays were evaluated in comparison with established standard RT-qPCR using samples from experimentally or field-infected animals. Even though all assays allowed detection of the target virus in less than 30 min, major differences were revealed concerning sensitivity, specificity, robustness, testing time, and complexity of assay design. These findings indicated that the success of an assay will depend on the integrated amplification technology. Therefore, the application-specific pros and cons of each method that were identified during this study provide very valuable insights for future development and optimization of pen-side tests.
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