ArticleBiology of reproduction2014
Epithelial basal cells are distinct from dendritic cells and macrophages in the mouse epididymis.
Article in Biology of reproduction, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
44 citing papers in PubMed, 71 citations in OpenAlex.
- Immunological dynamics of the epididymis: from experimental insights to clinical translation.Reproduction & fertility · 2026Review
- Depletion of CX3CR1Mucosal immunology · 2026Article
- Basal and Immune Cells of the Epididymis: An Electron Microscopy View of Their Association.Advances in experimental medicine and biology · 2025Review
- Innate Antiviral Defense of the Male Reproductive System.Advances in experimental medicine and biology · 2025Review
- Advancement and Potential Applications of Epididymal Organoids.Biomolecules · 2024Review
- Segmental differentiation of the murine epididymis: identification of segment-specific, GM1-enriched vesicles and regulation by luminal fluid factors†.Biology of reproduction · 2023Article
- Myd88 Signaling Is Involved in the Inflammatory Response in LPS-Induced Mouse Epididymitis and Bone-Marrow-Derived Dendritic Cells.International journal of molecular sciences · 2023Article
- Article
- The Role of Mononuclear Phagocytes in the Testes and Epididymis.International journal of molecular sciences · 2022Review
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- Epididymal epithelial degeneration and lipid metabolism impairment account for male infertility in occludin knockout mice.Frontiers in endocrinology · 2022Article
- Calcium Homeostasis in the Epididymal Microenvironment:Frontiers in cell and developmental biology · 2022Review
- Spatio-temporal landscape of mouse epididymal cells and specific mitochondria-rich segments defined by large-scale single-cell RNA-seq.Cell discovery · 2021Article
- Macrophage ubiquitin-specific protease 2 contributes to motility, hyperactivation, capacitation, and in vitro fertilization activity of mouse sperm.Cellular and molecular life sciences : CMLS · 2021Article
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- C-Type Natriuretic Peptide Plays an Anti-Inflammatory Role in Rat Epididymitis Induced byFrontiers in cellular and infection microbiology · 2021Article
- How bad is brazilian ginseng extract for reproductive parameters in mice?Histology and histopathology · 2020Article
- Article
- The expression and localization of V-ATPase and cytokeratin 5 during postnatal development of the pig epididymis.Asian-Australasian journal of animal sciences · 2020Article
- The Immune Characteristics of the Epididymis and the Immune Pathway of the Epididymitis Caused by Different Pathogens.Frontiers in immunology · 2020Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
The epithelium that lines the epididymal duct establishes the optimal milieu in which spermatozoa mature, acquire motility, and are stored. This finely tuned environment also protects antigenic sperm against pathogens and autoimmunity, which are potential causes of transient or permanent infertility. The epididymal epithelium is pseudostratified and contains basal cells (BCs) that are located beneath other epithelial cells. Previous studies showed that in the mouse epididymis, BCs possess macrophage-like characteristics. However, we previously identified a dense population of cells belonging to the mononuclear phagocyte (MP) system (comprised of macrophages and dendritic cells) in the basal compartment of the mouse epididymis and showed that a subset of MPs express the macrophage marker F4/80. In the present study, we evaluate the distribution of BCs and MPs in the epididymis of transgenic CD11c-EYFP mice, in which EYFP is expressed exclusively in MPs, using antibodies against the BC marker keratin 5 (KRT5) and the macrophage marker F4/80. Immunofluorescence labeling for laminin, a basement membrane marker, showed that BCs and most MPs are located in the basal region of the epithelium. Confocal microscopy showed that in the initial segment, both BCs and MPs project intraepithelial extensions and establish a very intricate network. Flow cytometry experiments demonstrated that epididymal MPs and BCs are phenotypically distinct. BCs do not express F4/80, and MPs do not express KRT5. Therefore, despite their proximity and some morphological similarities with peritubular macrophages and dendritic cells, BCs do not belong to the MP system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.