Evidence map›Paper›PMID 24648189›Full record

ArticleChembiochem : a European journal of chemical biology2014

Characterization of an environmental DNA-derived gene cluster that encodes the bisindolylmaleimide methylarcyriarubin.

Fang-Yuan Chang, Sean F Brady

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 26 citations in OpenAlex.

  1. Review
  2. Culture-independent discovery of natural products from soil metagenomes.Journal of industrial microbiology & biotechnology · 2016
    Review
  3. Article
  4. Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Fang-Yuan ChangLaboratory of Genetically Encoded Small Molecules, Howard Hughes Medical Institute, The Rockefeller University, 1230 York Avenue, New York, NY 10065 (USA).
Sean F Brady
Rockefeller University · US

Funding

Discovery and characterization of new natural products from uncultured bacteriaR01GM077516 · NIGMS · ROCKEFELLER UNIVERSITY · PI BRADY, SEAN F · 2006 to 2014
$3.0M
Howard Hughes Medical InstituteNIGMS NIH HHS GM-77516NIGMS NIH HHS R01 GM077516
6 · The paper itself

Abstract

Bisindolylmaleimides represent a naturally occurring class of metabolites that are of interest because of their protein kinase inhibition activity. From a metagenomic library constructed with soil DNA, we identified the four gene mar cluster, a bisindolylmaleimide gene cluster that encodes for methylarcyriarubin (1) production. Heterologous expression of the mar gene cluster in E. coli revealed that the Rieske dioxygenase MarC facilitates the oxidative decarboxylation of a chromopyrrolic acid (CPA) intermediate to yield the bisindolylmaleimide core. The characterization of the mar cluster defines a new role for CPA in the biosynthesis of structurally diverse bacterial tryptophan dimers.

Indexed as

Bacterial ProteinsCloning, MolecularDNAEscherichia coliGene LibraryIndolesMaleimidesMetagenomicsMultigene FamilyRecombinant ProteinsSoil MicrobiologyBacterial ProteinsbisindolylmaleimideDNAIndolesMaleimidesRecombinant Proteinsbiosynthesisbisindolylmaleimidesmetagenomicsnatural productsRieske dioxygenase

Identifiers

PMID24648189
PMCPMC4028703
OpenAlexW2022918716

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.