ArticleClinical oral investigations2015
Effect of interleukin-1β on ICAM-1 expression of dental pulp cells: role of PI3K/Akt, MEK/ERK, and cyclooxygenase.
Article in Clinical oral investigations, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 28 citations in OpenAlex.
- Nell-1 inhibits lipopolysaccharide-activated macrophages into M1 phenotype through the modulation of NF-κB pathway.Journal of molecular histology · 2025Article
- The genetic landscape of primary malignant melanoma of the cervix using integrated bioinformatics analysis and whole-exome sequencing.Frontiers in oncology · 2025Article
- Clustering human dental pulp fibroblasts spontaneously activate NLRP3 and AIM2 inflammasomes and induce IL-1β secretion.Regenerative therapy · 2024Article
- Inducing cyclooxygenase-2 expression, prostaglandin EJournal of dental sciences · 2024Article
- An in vitro anti-inflammatory effect of Thai propolis in human dental pulp cells.Journal of applied oral science : revista FOB · 2023Article
- Melatonin exerts anti-fibrinolytic effects by regulating IL-1β-induced changes in uPA, uPAR, and PAI-1 expression/production in human dental pulp cells.Journal of food and drug analysis · 2022Article
- Different expression patterns of inflammatory cytokines induced by lipopolysaccharides from Escherichia coli or Porphyromonas gingivalis in human dental pulp stem cells.BMC oral health · 2022Article
- [Anti-inflammatory and repaired effects of non-steroidal anti-inflammatory drugs on human dental pulp cells].Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences · 2020Article
- Contraction dynamics of dental pulp cell rod microtissues.Clinical oral investigations · 2020Article
- Aspirin modulates the inflammatory response in a thrombus‑stimulated LMVEC model.International journal of molecular medicine · 2018Article
- Interleukin-1β induces intercellular adhesion molecule-1 expression, thus enhancing the adhesion between mesenchymal stem cells and endothelial progenitor cells via the p38 MAPK signaling pathway.International journal of molecular medicine · 2018Article
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Authors and funding
9 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveInterleukin-1β (IL-1β) is an important inflammatory mediator of the dental pulp. IL-1β stimulates cyclooxygenase-2 (COX-2) expression and prostanoid production of pulp cells and affects the inflammatory and healing processes of the dental pulp. There are two interleukin-1 (IL-1) receptors, IL-1RI and IL-1RII, with opposing effect after activation. However, the expression of IL-1Rs, the effects of IL-1β on intercellular adhesion molecule-1 (ICAM-1) of dental pulp cells, and its relation to protein kinase B (Akt) signaling and COX activation are not clear.
methodHuman dental pulp cells were treated with IL-1β with/without pretreatment and co-incubation by LY294002 (a PI3K/Akt inhibitor), U0126 [a mitogen-activated protein kinase kinase/extracellular signal-regulated kinase (MEK/ERK) inhibitor], aspirin (a COX inhibitor), or eugenol (a COX inhibitor) for different time periods. The expression of ICAM-1, IL-1RI, and IL-1RII messenger RNA (mRNA) was evaluated by reverse transcriptase-polymerase chain reaction (RT-PCR). ICAM-1 protein expression was examined by western blotting. Soluble ICAM-1 (sICAM-1) level in the culture medium was determined by enzyme-linked immunosorbent assay (ELISA). Activation of Akt and ERK by IL-1β was measured by Pathscan p-Akt ELISA or western blot. Viable cell number was evaluated by 3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay.
resultsDental pulp cells expressed IL-1RI, but little IL-1RII. IL-1β stimulated COX-2 and ICAM-1 mRNA and protein expression as well as sICAM-1 production of pulp cells. Aspirin and eugenol enhanced the IL-1β-induced sICAM-1 production and ICAM-1 expression. IL-1β rapidly activated Akt and ERK. LY294002 and U0126 attenuated IL-1β-induced ICAM-1 expression and sICAM-1 production.
conclusionsThese results reveal that IL-1β may be involved in the pulpal inflammatory processes by stimulating ICAM-1 expression and secretion. These events are associated with IL-1RI expression and differential activation of PI3K/Akt and MEK/ERK and COX. CLINICAL RELEVANCE: Pharmacological inhibition of IL-1β, IL-1RI, COX-2, ICAM-1, and related signaling pathways (MEK/ERK and PI3K/Akt) may be useful for the control of pulpal inflammation.
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