ArticlePloS one2014
Porcupine is not required for the production of the majority of Wnts from primary human astrocytes and CD8+ T cells.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
16 citing papers in PubMed.
- Identification of the Wnt signal peptide that directs secretion on extracellular vesicles.Science advances · 2024Article
- A stromal lineage maintains crypt structure and villus homeostasis in the intestinal stem cell niche.BMC biology · 2023Article
- A Wnt-Independent LGR4-EGFR Signaling Axis in Cancer Metastasis.Cancer research · 2021Article
- WNT4 Balances Development vs Disease in Gynecologic Tissues and Women's Health.Endocrinology · 2021Review
- Selective Surface and Intraluminal Localization of Wnt Ligands on Small Extracellular Vesicles Released by HT-22 Hippocampal Neurons.Frontiers in cell and developmental biology · 2021Article
- Deletion of the Dishevelled family of genes disrupts anterior-posterior axis specification and selectively prevents mesoderm differentiation.Developmental biology · 2020Article
- Article
- Wnt family member 4 (WNT4) and WNT3A activate cell-autonomous Wnt signaling independent of porcupineThe Journal of biological chemistry · 2019Article
- Opposing actions of renal tubular- and myeloid-derived porcupine in obstruction-induced kidney fibrosis.Kidney international · 2019Article
- Wnt-11 as a Potential Prognostic Biomarker and Therapeutic Target in Colorectal Cancer.Cancers · 2019Article
- Article
- WNT Signaling in Cardiac and Vascular Disease.Pharmacological reviews · 2018Review
- Winding back Wnt signalling: potential therapeutic targets for treating gastric cancers.British journal of pharmacology · 2017Review
- Review
- Migration of CD8+ T Cells into the Central Nervous System Gives Rise to Highly Potent Anti-HIV CD4dimCD8bright T Cells in a Wnt Signaling-Dependent Manner.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- Distinct requirements for Wntless in habenular development.Developmental biology · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Wnts are small secreted glycoproteins that are highly conserved among species. To date, 19 Wnts have been described, which initiate a signal transduction cascade that is either β-catenin dependent or independent, culminating in the regulation of hundreds of target genes. Extracellular release of Wnts is dependent on lipidation of Wnts by porcupine, a membrane-bound-O-acyltransferase protein in the endoplasmic reticulum. Studies demonstrating the requirement of porcupine for Wnts production are based on cell line and non-human primary cells. We evaluated the requirement for porcupine for Wnts production in human primary astrocytes and CD8+ T cells. Using IWP-2, an inhibitor of porcupine, or siRNA targeting porcupine, we demonstrate that porcupine is not required for the release of Wnt 1, 3, 5b, 6,7a, 10b, and 16a. While IWP had no effect on Wnt 2b release, knockdown of porcupine by siRNA reduced Wnt 2b release by 60%. These data indicate that porcupine-mediated production of Wnts is context dependent and is not required for all Wnts production, suggesting that alternative mechanisms exist for Wnts production.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.