ArticlePloS one2014
Apigenin induces the apoptosis and regulates MAPK signaling pathways in mouse macrophage ANA-1 cells.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- Suppression of Inflammatory Responses Through GD3 Synthase (GD3S) Silencing Mediated by TLR4 Signaling: In Vitro and In Silico Analysis.Molecular neurobiology · 2026Article
- Apigenin supplementation substantially improves rooster sperm freezability and post-thaw function.Scientific reports · 2024Article
- Review
- Apigenin Modulates AnxA6- and TNAP-Mediated Osteoblast Mineralization.International journal of molecular sciences · 2022Article
- The Potential Role of Apigenin in Cancer Prevention and Treatment.Molecules (Basel, Switzerland) · 2022Review
- The Biphasic Effect of Flavonoids on Oxidative Stress and Cell Proliferation in Breast Cancer Cells.Antioxidants (Basel, Switzerland) · 2022Article
- Flavonoids: New Frontier for Immuno-Regulation and Breast Cancer Control.Antioxidants (Basel, Switzerland) · 2019Review
- Apigenin Inhibits IL-31 Cytokine in Human Mast Cell and Mouse Skin Tissues.Molecules (Basel, Switzerland) · 2019Article
- Potential Role of Flavonoids in Treating Chronic Inflammatory Diseases with a Special Focus on the Anti-Inflammatory Activity of Apigenin.Antioxidants (Basel, Switzerland) · 2019Review
- The significant impact of apigenin on different aspects of autoimmune disease.Inflammopharmacology · 2018Review
- Apigenin, a dietary flavonoid, induces apoptosis, DNA damage, and oxidative stress in human breast cancer MCF-7 and MDA MB-231 cells.Naunyn-Schmiedeberg's archives of pharmacology · 2018Article
- Luteolin Induces Apoptosis and Autophagy in Mouse Macrophage ANA-1 Cells via the Bcl-2 Pathway.Journal of immunology research · 2018Article
- Immunomodulatory effects of cigarette smoke condensate in mouse macrophage cell line.International journal of immunopathology and pharmacology · 2017Article
- Selective Toxicity of Non Polar Bioactive Compounds of Persian Gulf Sea Squirt Phallusia Nigra on Skin Mitochondria Isolated from Rat Model of MelanomaAsian Pacific journal of cancer prevention : APJCP · 2017Article
- Article
- Apigenin protects against bleomycin-induced lung fibrosis in rats.Experimental and therapeutic medicine · 2016Article
- Selective Toxicity of Persian Gulf Sea Cucumber (Holothuria parva) and Sponge (Haliclona oculata) Methanolic Extracts on Liver Mitochondria Isolated from an Animal Model of Hepatocellular Carcinoma.Hepatitis monthly · 2015Article
- Apigenin promotes osteogenic differentiation of human mesenchymal stem cells through JNK and p38 MAPK pathways.Molecular and cellular biochemistry · 2015Article
- Anti-Proliferative Effect of Rosmarinus officinalis L. Extract on Human Melanoma A375 Cells.PloS one · 2015Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Apigenin is a naturally occurring plant flavonoid that possesses antioxidant, anti-cancer and anti-inflammatory properties. However, there are few reports has been done on the ability of apigenin to induce apoptosis in macrophages. In this study, mouse macrophage ANA-1 cells were incubated with different concentrations of apigenin. The cell viability was determined by an MTT assay. The cell apoptosis were analyzed by flow cytometric analysis. Apoptosis were also analyzed using a TUNEL assay and a DNA ladder. The level of intracellular ROS was detected using a dichlorofluorescein -diacetate probe. The expression levels of apoptosis-related proteins were detected by western blot analysis. The results showed that apigenin decreased the viability of ANA-1 cells and induced apoptosis in a dose- and time-dependent manner. Apigenin increased the level of intracellular ROS, downregulated the expression of Bcl-2 and upregulated the expression of caspase-3 and caspase-8 in ANA-1 cells. Furthermore, apigenin downregulated the expression of phospho-ERK and phospho-JNK, upregulated the expression of phospho-p38 and had no significant effect on the expression of Bax, ERK, JNK and p38. The results suggested that apigenin induced cell apoptosis in mouse macrophage ANA-1 cells may via increasing intracellular ROS, regulating the MAPK pathway, and then inhibiting Bcl-2 expression.
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