ArticleBMC genomics2014
Identification, expression and interaction analyses of calcium-dependent protein kinase (CPK) genes in canola (Brassica napus L.).
Article in BMC genomics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 79 citations in OpenAlex.
- Calcium-dependent protein kinases as central hubs in plant abiotic stress signaling: mechanisms and prospects for crop improvement.Frontiers in plant science · 2025Review
- Calcium Signaling and the Response to Heat Shock in Crop Plants.International journal of molecular sciences · 2023Review
- Article
- Genome-Wide Survey Indicates Diverse Physiological Roles ofInternational journal of molecular sciences · 2022Article
- Genome-wide survey of Calcium-Dependent Protein Kinases (CPKs) in fiveFrontiers in plant science · 2022Article
- Delineating Calcium Signaling Machinery in Plants: Tapping the Potential through Functional Genomics.Current genomics · 2021Review
- Omics: The way forward to enhance abiotic stress tolerance inGM crops & food · 2021Review
- Turnip mosaic virus in oilseed rape activates networks of sRNA-mediated interactions between viral and host genomes.Communications biology · 2020Article
- Engineering Multiple Abiotic Stress Tolerance in Canola,Frontiers in plant science · 2020Review
- Expression, Subcellular Localization, and Interactions of CPK Family Genes in Maize.International journal of molecular sciences · 2019Article
- HTRgene: a computational method to perform the integrated analysis of multiple heterogeneous time-series data: case analysis of cold and heat stress response signaling genes in Arabidopsis.BMC bioinformatics · 2019Article
- Insights on Calcium-Dependent Protein Kinases (CPKs) Signaling for Abiotic Stress Tolerance in Plants.International journal of molecular sciences · 2019Review
- Calcium-Dependent Protein Kinase Genes inMolecules (Basel, Switzerland) · 2019Article
- Calcium Signaling-Mediated Plant Response to Cold Stress.International journal of molecular sciences · 2018Review
- Proteomic Analysis of Rapeseed Root Response to Waterlogging Stress.Plants (Basel, Switzerland) · 2018Article
- Article
- Genome-wide survey indicates diverse physiological roles of the turnip (Brassica rapa var. rapa) calcium-dependent protein kinase genes.Scientific reports · 2017Article
- Genome-wide survey indicates diverse physiological roles of the barley (Hordeum vulgare L.) calcium-dependent protein kinase genes.Scientific reports · 2017Article
- The calcium-dependent protein kinase (CDPK) and CDPK-related kinase gene families inFEBS open bio · 2017Article
- Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCanola (Brassica napus L.) is one of the most important oil-producing crops in China and worldwide. The yield and quality of canola is frequently threatened by environmental stresses including drought, cold and high salinity. Calcium is a well-known ubiquitous intracellular secondary messenger in plants. Calcium-dependent protein kinases (CPKs) are Ser/Thr protein kinases found only in plants and some protozoans. CPKs are Ca2+ sensors that have both Ca2+ sensing function and kinase activity within a single protein and play crucial roles in plant development and responses to various environmental stresses.
resultsIn this study, we mined the available expressed sequence tags (ESTs) of B. napus and identified a total of 25 CPK genes, among which cDNA sequences of 23 genes were successfully cloned from a double haploid cultivar of canola. Phylogenetic analysis demonstrated that they could be clustered into four subgroups. The subcellular localization of five selected BnaCPKs was determined using green fluorescence protein (GFP) as the reporter. Furthermore, the expression levels of 21 BnaCPK genes in response to salt, drought, cold, heat, abscisic acid (ABA), low potassium (LK) and oxidative stress were studied by quantitative RT-PCR and were found to respond to multiple stimuli, suggesting that canola CPKs may be convergence points of different signaling pathways. We also identified and cloned five and eight Clade A basic leucine zipper (bZIP) and protein phosphatase type 2C (PP2C) genes from canola and, using yeast two-hybrid and bimolecular fluorescence complementation (BiFC), determined the interaction between individual BnaCPKs and BnabZIPs or BnaPP2Cs (Clade A). We identified novel, interesting interaction partners for some of the BnaCPK proteins.
conclusionWe present the sequences and characterization of CPK gene family members in canola for the first time. This work provides a foundation for further crop improvement and improved understanding of signal transduction in plants.
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