ArticleBMC microbiology2014
Copper tolerance mediated by polyphosphate degradation and low-affinity inorganic phosphate transport system in Escherichia coli.
Article in BMC microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 48 citations in OpenAlex.
- The Synergy Between a Silver-Ruthenium Antimicrobial and Aminoglycosides Is Based on Severe Macromolecular Damage.Molecular microbiology · 2026Article
- Single-cell ionomes of terrestrial cryosphere algae.Communications earth & environment · 2026Article
- BR-bodies facilitate adaptive responses and survival during copper stress in Caulobacter crescentus.The Journal of biological chemistry · 2025Article
- Role of Polyphosphate as an Inorganic Chaperone to Prevent Protein Aggregation Under Copper Stress inMicroorganisms · 2024Article
- Synthetic bacteria designed using ars operons: a promising solution for arsenic biosensing and bioremediation.World journal of microbiology & biotechnology · 2024Review
- Inorganic phosphate modifies stationary phase fitness and metabolic pathways inFrontiers in microbiology · 2024Article
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- Inorganic Additive Improves the Survival of the Probiotic Lacticaseibacillus rhamnosus CRL1505 During Spray Drying, Rehydration, and Storage.Current microbiology · 2021Article
- The biology of thermoacidophilic archaea from the order Sulfolobales.FEMS microbiology reviews · 2021Article
- Model systems for studying polyphosphate biology: a focus on microorganisms.Current genetics · 2021Review
- Dynamic Polyphosphate Metabolism Coordinating with Manganese Ions Defends against Oxidative Stress in the Extreme Bacterium Deinococcus radiodurans.Applied and environmental microbiology · 2021Article
- Transcriptional Responses ofFrontiers in microbiology · 2021Article
- Interactions between DksA and Stress-Responsive Alternative Sigma Factors Control Inorganic Polyphosphate Accumulation in Escherichia coli.Journal of bacteriology · 2020Article
- Disruption of Phosphate Homeostasis Sensitizes Staphylococcus aureus to Nutritional Immunity.Infection and immunity · 2020Article
- Inorganic Polyphosphate Accumulation in Escherichia coli Is Regulated by DksA but Not by (p)ppGpp.Journal of bacteriology · 2019Article
- [A fluorometric method for direct detection of inorganic polyphosphate in enterohemorrhagicNan fang yi ke da xue xue bao = Journal of Southern Medical University · 2019Article
- Intracellular Polyphosphate Levels in Gluconacetobacter diazotrophicus Affect Tolerance to Abiotic Stressors and Biofilm Formation.Microbes and environments · 2018Article
- Inorganic polyphosphates and heavy metal resistance in microorganisms.World journal of microbiology & biotechnology · 2018Review
- Mutations in Escherichia coli Polyphosphate Kinase That Lead to Dramatically IncreasedJournal of bacteriology · 2018Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMetal tolerance in bacteria has been related to polyP in a model in which heavy metals stimulate the polymer hydrolysis, forming metal-phosphate complexes that are exported. As previously described in our laboratory, Escherichia coli cells grown in media containing a phosphate concentration >37 mM maintained an unusually high polyphosphate (polyP) level in stationary phase. The aim of the present work was to evaluate the influence of polyP levels as the involvement of low-affinity inorganic phosphate transport (Pit) system in E. coli copper tolerance.
resultsPolyP levels were modulated by the media phosphate concentration and/or using mutants in polyP metabolism. Stationary phase wild-type cells grown in high phosphate medium were significantly more tolerant to copper than those grown in sufficient phosphate medium. Copper addition to tolerant cells induced polyP degradation by PPX (an exopolyphosphatase), phosphate efflux and membrane polarization. ppk-ppx- (unable to synthesize/degrade polyP), ppx- (unable to degrade polyP) and Pit system mutants were highly sensitive to metal even in high phosphate media. In exponential phase, CopA and polyP-Pit system would act simultaneously to detoxify the metal or one could be sufficient to safeguard the absence of the other.
conclusionsOur results support a mechanism for copper detoxification in exponential and stationary phases of E. coli, involving Pit system and degradation of polyP. Data reflect the importance of the environmental phosphate concentration in the regulation of the microbial physiological state.
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