ArticleBMC microbiology2014
Identification of a predicted partner-switching system that affects production of the gene transfer agent RcGTA and stationary phase viability in Rhodobacter capsulatus.
Article in BMC microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- Article
- Control of a gene transfer agent cluster in Caulobacter crescentus by transcriptional activation and anti-termination.Nature communications · 2024Article
- Function of the RNA-targeting class 2 type VI CRISPR Cas system ofFrontiers in microbiology · 2024Article
- Computational and cellular exploration of the protein-protein interaction betweenCommunicative & integrative biology · 2023Article
- Prophage-like gene transfer agents promote Caulobacter crescentus survival and DNA repair during stationary phase.PLoS biology · 2022Article
- Article
- STAS Domain Only Proteins in Bacterial Gene Regulation.Frontiers in cellular and infection microbiology · 2021Review
- Connection Between Chromosomal Location and Function of CtrA Phosphorelay Genes in Alphaproteobacteria.Frontiers in microbiology · 2021Article
- Cyclic di-GMP-Mediated Regulation of Gene Transfer and Motility in Rhodobacter capsulatus.Journal of bacteriology · 2020Article
- Gene Transfer Agents in Symbiotic Microbes.Results and problems in cell differentiation · 2020Review
- Identification and characterization of a direct activator of a gene transfer agent.Nature communications · 2019Article
- Integrated Transcriptional Regulatory Network of Quorum Sensing, Replication Control, and SOS Response inFrontiers in microbiology · 2019Article
- The Protease ClpXP and the PAS Domain Protein DivL Regulate CtrA and Gene Transfer Agent Production in Rhodobacter capsulatus.Applied and environmental microbiology · 2018Article
- Insights into origin and evolution of α-proteobacterial gene transfer agents.Virus evolution · 2017Article
- Functional and Evolutionary Characterization of a Gene Transfer Agent's Multilocus "Genome".Molecular biology and evolution · 2016Article
- Mobile genetic elements: in silico, in vitro, in vivo.Molecular ecology · 2016Article
- Gene co-expression network analysis in Rhodobacter capsulatus and application to comparative expression analysis of Rhodobacter sphaeroides.BMC genomics · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundProduction of the gene transfer agent RcGTA in the α-proteobacterium Rhodobacter capsulatus is dependent upon the response regulator protein CtrA. Loss of this regulator has widespread effects on transcription in R. capsulatus, including the dysregulation of numerous genes encoding other predicted regulators. This includes a set of putative components of a partner-switching signaling pathway with sequence homology to the σ-regulating proteins RsbV, RsbW, and RsbY that have been extensively characterized for their role in stress responses in gram-positive bacteria. These R. capsulatus homologues, RbaV, RbaW, and RbaY, have been investigated for their possible role in controlling RcGTA gene expression.
resultsA mutant strain lacking rbaW showed a significant increase in RcGTA gene expression and production. Mutation of rbaV or rbaY led to a decrease in RcGTA gene expression and production, and these mutants also showed decreased viability in the stationary phase and produced unusual colony morphologies. In vitro and in vivo protein interaction assays demonstrated that RbaW and RbaV interact. A combination of gene disruptions and protein-protein interaction assays were unsuccessful in attempts to identify a cognate σ factor, and the genetic data support a model where the RbaV protein that is the determinant regulator of RcGTA gene expression in this system.
conclusionsThese findings provide new information about RcGTA regulation by a putative partner-switching system and further illustrate the integration of RcGTA production into R. capsulatus physiology.
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