ArticleInvestigative ophthalmology & visual science2014
Pathogenicity of ocular isolates of Acinetobacter baumannii in a mouse model of bacterial endophthalmitis.
Article in Investigative ophthalmology & visual science, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 24 citations in OpenAlex.
- Electroretinographic Evaluations of Eyes With Endophthalmitis.Translational vision science & technology · 2024Article
- Oral administration of S-nitroso-L-glutathione (GSNO) provides anti-inflammatory and cytoprotective effects during ocular bacterial infections.Cellular and molecular life sciences : CMLS · 2023Article
- Transcriptomics and systems biology identify non-antibiotic drugs for the treatment of ocular bacterial infection.iScience · 2022Article
- Essential Role of NLRP3 Inflammasome in Mediating IL-1β Production and the Pathobiology of Staphylococcus aureus Endophthalmitis.Infection and immunity · 2022Article
- Gram-Negative Bacteria Holding Together in a Biofilm: TheMicroorganisms · 2021Review
- Transcriptomic and Histological Analysis of Exacerbated Immune Response in Multidrug-ResistantFrontiers in immunology · 2021Article
- Bacterial Burden Declines But Neutrophil Infiltration and Ocular Tissue Damage Persist in ExperimentalFrontiers in cellular and infection microbiology · 2021Article
- Aging, But Not Sex and Genetic Diversity, Impacts the Pathobiology of Bacterial Endophthalmitis.Investigative ophthalmology & visual science · 2020Article
- Evaluation of Susceptibility and Innate Immune Response in C57BL/6 and BALB/c Mice During Candida albicans Endophthalmitis.Investigative ophthalmology & visual science · 2020Article
- Zebrafish are Resistant toPathogens (Basel, Switzerland) · 2019Article
- Pathobiology ofMicroorganisms · 2019Article
- Zika virus infects cells lining the blood-retinal barrier and causes chorioretinal atrophy in mouse eyes.JCI insight · 2017Article
- 5-Aminoimidazole-4-carboxamide ribonucleoside-mediated adenosine monophosphate-activated protein kinase activation induces protective innate responses in bacterial endophthalmitis.Cellular microbiology · 2016Article
- Modeling intraocular bacterial infections.Progress in retinal and eye research · 2016Review
- Protective Effect of a Synbiotic against Multidrug-Resistant Acinetobacter baumannii in a Murine Infection Model.Antimicrobial agents and chemotherapy · 2016Article
- Temporal retinal transcriptome and systems biology analysis identifies key pathways and hub genes in Staphylococcus aureus endophthalmitis.Scientific reports · 2016Article
- Mitochondria mediates caspase-dependent and independent retinal cell death in Staphylococcus aureus endophthalmitis.Cell death discovery · 2016Article
- In Vivo Role of TLR2 and MyD88 Signaling in Eliciting Innate Immune Responses in Staphylococcal Endophthalmitis.Investigative ophthalmology & visual science · 2015Article
- Article
- Retinal photoreceptor expresses toll-like receptors (TLRs) and elicits innate responses following TLR ligand and bacterial challenge.PloS one · 2015Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
purposeTo determine the virulence properties of ocular isolates of Acinetobacter baumannii in causing endophthalmitis in a mouse model.
methodsEndophthalmitis was induced by intravitreal injections of the bacteria into C57BL/6 (B6) mouse eyes. The disease progression was monitored by ophthalmoscopic, electroretinography (ERG), histologic, cell death (TUNEL labeling), and microbiological parameters. The expression of cytokines/chemokines was checked by quantitative RT-PCR (qRT-PCR) and ELISA. Flow cytometry was used to determine cellular infiltration. The role of neutrophils was determined using neutropenic mice. The virulence traits (biofilm formation, adherence, and cytotoxicity) of the ocular isolates were tested using corneal epithelial cells.
resultsAmong the three clinical isolates and a standard ATCC 19606 strain tested, a biofilm producing multidrug resistant (MDR) strain of A. baumannii AB12 caused severe endophthalmitis (100% destruction of the eyes) leading to the loss of retinal function as assessed by ERG analysis. Elevated levels of inflammatory mediators (TNF-α, IL-1β, CXCL2, and IL-6) were detected in AB12-infected eyes. Histologic and TUNEL staining revealed increased retinal cell death and the flow cytometry data showed the presence of inflammatory cells, primarily neutrophils (CD45(+)/Ly6G(+)). Neutropenic mice showed an increased bacterial burden, reduced inflammatory response, and severe tissue destruction.
conclusionsThese results indicate that A. baumannii causes severe intraocular inflammation and retinal damage. Furthermore, neutrophils play an important role in the pathogenesis of A. baumannii endophthalmitis.
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Registered trials
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