ArticleLasers in surgery and medicine2014
Heat stress induced cell death mechanisms in hepatocytes and hepatocellular carcinoma: in vitro and in vivo study.
Article in Lasers in surgery and medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Temporal dynamics of thermal warming in brown trout hepatocyte spheroids: ultrastructural and immunocytochemical evidence of cellular remodelling.Cell and tissue research · 2026Article
- Passive Acoustic Dynamic Differentiation and Mapping (PADAM): A Time-Domain Passive Cavitation Localization and Classification Approach.IEEE transactions on bio-medical engineering · 2026Article
- High Temperature and Ethinylestradiol May Reduce Body Growth, Liver and Hepatocyte Volumes and Lipid Droplets in Adult Male Guppies.Animals : an open access journal from MDPI · 2025Article
- Radiosensitization Induced by Magnetic Hyperthermia of PEGylated Nickel Ferrite Nanoparticles on Breast Cancer Cells.International journal of molecular sciences · 2025Article
- The XIAP inhibitor AZD5582 improves the treatment effect of microwave ablation on hepatocellular carcinoma.Frontiers in immunology · 2025Article
- Review
- Prx4 acts as DAMP in shrimp, enhancing bacterial resistance via the toll pathway and prophenoloxidase activation.iScience · 2023Article
- Hyperthermia-induced changes in leukocyte survival and phagocytosis: a comparative study in bovine and buffalo leukocytes.Frontiers in veterinary science · 2023Article
- Experiments on physical ablation of long bone using microwave ablation; defining optimal settings using ex- and in-vivo experiments.PloS one · 2023Article
- Effect of Dietary 4-Phenylbuthyric Acid Supplementation on Acute Heat-Stress-Induced Hyperthermia in Broiler Chickens.Animals : an open access journal from MDPI · 2022Article
- Duodenal Metabolic Profile Changes in Heat-Stressed Broilers.Animals : an open access journal from MDPI · 2022Article
- Impact of Heat Stress on Bovine Sperm Quality and Competence.Animals : an open access journal from MDPI · 2022Review
- Altered relationship between gluconeogenesis and immunity in broilers exposed to heat stress for different durations.Poultry science · 2021Article
- Hepatic lipid metabolomics in response to heat stress in local broiler chickens breed (Huaixiang chickens).Veterinary medicine and science · 2021Article
- Advances in Nanomaterial-Mediated Photothermal Cancer Therapies: Toward Clinical Applications.Biomedicines · 2021Review
- International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group · 2020Review
- Mathematical modeling of mass and energy transport for thermoembolization.International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group · 2020Article
- Assessment of hepatocellular carcinoma treatment response with LI-RADS: a pictorial review.Insights into imaging · 2019Review
- Single-Dose Neoadjuvant AKT Pathway Inhibitor Reduces Growth of Hepatocellular Carcinoma after Laser Thermal Ablation in Small-Animal Model.Radiology · 2019Article
- Heat Stress and Thermal Ablation Induce Local Expression of Nerve Growth Factor Inducible (VGF) in Hepatocytes and Hepatocellular Carcinoma: Preclinical and Clinical Studies.Gene expression · 2018Article
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Authors and funding
7 authors.
Funding
Abstract
BACKGROUND AND
objectiveThe aims of the present study were to investigate the thermal-dose dependent effect of heat stress on hepatocyte and HCC cell death mechanisms using clinically relevant experimental heat stress conditions in vitro and to investigate apoptotic cell death induced by laser thermal ablation in vivo. STUDY DESIGN/MATERIALS AND
methodsInstitutional Animal Care and Use Committee approved all studies. Hepatocyte and HCC cell lines were heat stressed from 37 to 60°C for 2 or 10 minutes and assessed for viability, cytotoxicity and caspase-3/7 activity at 6 and/or 24 hours post-treatment (N = 3). Viability experiments were repeated with the RIPK1 inhibitor Necrostatin-1 to block necroptosis (N = 3). Rats with orthotopic HCC tumors stably expressing luciferase (N1S1luc2) were randomized to US-guided laser ablation (3W-45s for an intentional partial ablation; N = 6) or sham (N = 6) and followed by post-ablation caspase-3/7 bioluminescence imaging at 6 and 24 hours and cleaved caspase-3 immunostaining. P < 0.05 was considered statistically significant.
resultsHeat-stress induced apoptosis and necrosis in hepatocytes and HCC cells in a thermal dose and cell-type dependent manner. Inhibition of RIPIK1-mediated necroptosis induced a significant, differential increase in HCC cell viability under physiologic and hyperthermic heat stress (P < 0.001). Intentional partial laser thermal ablation induced a significant increase in caspase-3/7 activity in the laser versus sham ablation groups at both 6 hours (10.1-fold, P < 0.01) and 24 hours (16.7-fold, P < 0.02). Immunohistochemistry confirmed increased cleaved caspase-3 staining at the tumor ablation margin 24 hours post-ablation.
conclusionsBoth regulated and non-regulated cell death mechanisms mediate heat stress-induced HCC cell killing and vary between hepatocytes and HCC subtypes. Apoptosis is a significant mechanism of cell death at the HCC tumor ablation margin.
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