ArticlePloS one2014
True-breeding targeted gene knock-out in barley using designer TALE-nuclease in haploid cells.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 108 citations in OpenAlex.
- Enhancing tiny millets through genome editing: current status and future prospects.Molecular genetics and genomics : MGG · 2025Review
- CRISPR/Cas technology: fueling the future of Biofuel production with sugarcane.Functional & integrative genomics · 2024Review
- Opportunities and Challenges of In Vitro Tissue Culture Systems in the Era of Crop Genome Editing.International journal of molecular sciences · 2023Review
- CRISPR-Cas engineering in food science and sustainable agriculture: recent advancements and applications.Bioprocess and biosystems engineering · 2023Review
- Genome Editing and Designer Crops for the Future.Methods in molecular biology (Clifton, N.J.) · 2022Article
- A CRISPR way for accelerating cereal crop improvement: Progress and challenges.Frontiers in genetics · 2022Review
- A Critical Review: Recent Advancements in the Use of CRISPR/Cas9 Technology to Enhance Crops and Alleviate Global Food Crises.Current issues in molecular biology · 2021Review
- Targeted genome modifications in cereal crops.Breeding science · 2021Article
- Genome editing in cereal crops: an overview.Transgenic research · 2021Review
- Simultaneous TALEN-mediated knockout of chrysanthemum DMC1 genes confers male and female sterility.Scientific reports · 2020Article
- Article
- Modern Trends in Plant Genome Editing: An Inclusive Review of the CRISPR/Cas9 Toolbox.International journal of molecular sciences · 2019Review
- Cas Endonuclease Technology-A Quantum Leap in the Advancement of Barley and Wheat Genetic Engineering.International journal of molecular sciences · 2019Review
- Programmable Molecular Scissors: Applications of a New Tool for Genome Editing in Biotech.Molecular therapy. Nucleic acids · 2019Review
- TALEN-mediated targeted mutagenesis of more than 100 COMT copies/alleles in highly polyploid sugarcane improves saccharification efficiency without compromising biomass yield.Plant biotechnology journal · 2018Article
- A simple and efficient CRISPR/Cas9 platform for induction of single and multiple, heritable mutations in barley (Plant methods · 2018Article
- Evaluation of the mature grain phytase candidate HvPAPhy_a gene in barley (Hordeum vulgare L.) using CRISPR/Cas9 and TALENs.Plant molecular biology · 2017Article
- Genome Engineering and Agriculture: Opportunities and Challenges.Progress in molecular biology and translational science · 2017Review
- Aquatic Plant Genomics: Advances, Applications, and Prospects.International journal of genomics · 2017Review
- Plant genome editing with TALEN and CRISPR.Cell & bioscience · 2017Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription activator-like effector nucleases (TALENs) are customizable fusion proteins able to cleave virtually any genomic DNA sequence of choice, and thereby to generate site-directed genetic modifications in a wide range of cells and organisms. In the present study, we expressed TALENs in pollen-derived, regenerable cells to establish the generation of instantly true-breeding mutant plants. A gfp-specific TALEN pair was expressed via Agrobacterium-mediated transformation in embryogenic pollen of transgenic barley harboring a functional copy of gfp. Thanks to the haploid nature of the target cells, knock-out mutations were readily detected, and homozygous primary mutant plants obtained following genome duplication. In all, 22% of the TALEN transgenics proved knocked out with respect to gfp, and the loss of function could be ascribed to the deletions of between four and 36 nucleotides in length. The altered gfp alleles were transmitted normally through meiosis, and the knock-out phenotype was consistently shown by the offspring of two independent mutants. Thus, here we describe the efficient production of TALEN-mediated gene knock-outs in barley that are instantaneously homozygous and non-chimeric in regard to the site-directed mutations induced. This TALEN approach has broad applicability for both elucidating gene function and tailoring the phenotype of barley and other crop species.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.