ArticlePloS one2014
Isl1 and Pou4f2 form a complex to regulate target genes in developing retinal ganglion cells.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- isl1a coordinates onset and termination of regeneration of the zebrafish lateral line.NPJ Regenerative medicine · 2026Article
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- Tbr2-dependent parallel pathways regulate the development of distinct ipRGC subtypes.Communications biology · 2026Article
- Risk of Sperm Disorders and Impaired Fertility in Frozen-Thawed Bull Semen: A Genome-Wide Association Study.Animals : an open access journal from MDPI · 2024Article
- The landscape of the long non-coding RNAs in developing mouse retinas.BMC genomics · 2023Article
- Key transcription factors influence the epigenetic landscape to regulate retinal cell differentiation.Nucleic acids research · 2023Article
- Pou4f1-Tbr1 transcriptional cascade controls the formation of Jam2-expressing retinal ganglion cells.Frontiers in ophthalmology · 2023Article
- Article
- Evidence for ceramide induced cytotoxicity in retinal ganglion cells.Experimental eye research · 2021Article
- Phosphorylation of islet-1 serine 269 by CDK1 increases its transcriptional activity and promotes cell proliferation in gastric cancer.Molecular medicine (Cambridge, Mass.) · 2021Article
- Genetic control of retinal ganglion cell genesis.Cellular and molecular life sciences : CMLS · 2021Review
- Contrasting DNA-binding behaviour by ISL1 and LHX3 underpins differential gene targeting in neuronal cell specification.Journal of structural biology: X · 2021Article
- Single Cell Transcriptomic Analyses Reveal the Impact of bHLH Factors on Human Retinal Organoid Development.Frontiers in cell and developmental biology · 2021Article
- Quick Commitment and Efficient Reprogramming Route of Direct Induction of Retinal Ganglion Cell-like Neurons.Stem cell reports · 2020Article
- On the Generation and Regeneration of Retinal Ganglion Cells.Frontiers in cell and developmental biology · 2020Article
- Adenosine receptor expression in the adult zebrafish retina.Purinergic signalling · 2019Article
- Systematic identification of intergenic long-noncoding RNAs in mouse retinas using full-length isoform sequencing.BMC genomics · 2019Article
- Common and distinct transcriptional signatures of mammalian embryonic lethality.Nature communications · 2019Article
- Article
- Islet1 and Brn3 Expression Pattern Study in Human Retina and hiPSC-Derived Retinal Organoid.Stem cells international · 2019Article
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Authors and funding
6 authors.
Funding
Abstract
Precise regulation of gene expression during biological processes, including development, is often achieved by combinatorial action of multiple transcription factors. The mechanisms by which these factors collaborate are largely not known. We have shown previously that Isl1, a Lim-Homeodomain transcription factor, and Pou4f2, a class IV POU domain transcription factor, co-regulate a set of genes required for retinal ganglion cell (RGC) differentiation. Here we further explore how these two factors interact to precisely regulate gene expression during RGC development. By GST pulldown assays, co-immunoprecipitation, and electrophoretic mobility shift assays, we show that Isl1 and Pou4f2 form a complex in vitro and in vivo, and identify the domains within these two proteins that are responsible for this interaction. By luciferase assay, in situ hybridization, and RNA-seq, we further demonstrate that the two factors contribute quantitatively to gene expression in the developing RGCs. Although each factor alone can activate gene expression, both factors are required to achieve optimal expression levels. Finally, we discover that Isl1 and Pou4f2 can interact with other POU and Lim-Homeodomain factors respectively, indicating the interactions between these two classes of transcription factors are prevalent in development and other biological processes.
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