ArticlePloS one2014
Sequencing and characterisation of rearrangements in three S. pastorianus strains reveals the presence of chimeric genes and gives evidence of breakpoint reuse.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
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- High-quality haplotype-resolved genome assembly of cultivated octoploid strawberry.Horticulture research · 2023Article
- Identification of European isolates of the lager yeast parent Saccharomyces eubayanus.FEMS yeast research · 2022Article
- Aneuploidy influences the gene expression profiles in Saccharomyces pastorianus group I and II strains during fermentation.PLoS genetics · 2022Article
- Meiotic Chromosomal Abnormality Detected in a Heterozygote ofFrontiers in plant science · 2022Article
- Transcriptional Profile of the Industrial Hybrid Saccharomyces pastorianus Reveals Temperature-Dependent Allele Expression Bias and Preferential Orthologous Protein Assemblies.Molecular biology and evolution · 2021Article
- Interspecific hybridization as a driver of fungal evolution and adaptation.Nature reviews. Microbiology · 2021Review
- Packing a punch: understanding how flavours are produced in lager fermentations.FEMS yeast research · 2021Review
- ODFM, an omics data resource from microorganisms associated with fermented foods.Scientific data · 2021Article
- HybridMine: A Pipeline for Allele Inheritance and Gene Copy Number Prediction in Hybrid Genomes and Its Application to Industrial Yeasts.Microorganisms · 2020Article
- An update on the diversity, ecology and biogeography of the Saccharomyces genus.FEMS yeast research · 2020Review
- Plasticity of Mitochondrial DNA Inheritance and its Impact on Nuclear Gene Transcription in Yeast Hybrids.Microorganisms · 2020Article
- Genomic Adaptation ofFrontiers in genetics · 2020Review
- Improving Industrially Relevant Phenotypic Traits by Engineering Chromosome Copy Number inFrontiers in genetics · 2020Article
- Chromosome level assembly and comparative genome analysis confirm lager-brewing yeasts originated from a single hybridization.BMC genomics · 2019Article
- Fermentation innovation through complex hybridization of wild and domesticated yeasts.Nature ecology & evolution · 2019Article
- Lager-brewing yeasts in the era of modern genetics.FEMS yeast research · 2019Review
- Improvement of thermotolerance in Lachancea thermotolerans using a bacterial selection pressure.Journal of industrial microbiology & biotechnology · 2019Article
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4 authors.
Funding
Abstract
Gross chromosomal rearrangements have the potential to be evolutionarily advantageous to an adapting organism. The generation of a hybrid species increases opportunity for recombination by bringing together two homologous genomes. We sought to define the location of genomic rearrangements in three strains of Saccharomyces pastorianus, a natural lager-brewing yeast hybrid of Saccharomyces cerevisiae and Saccharomyces eubayanus, using whole genome shotgun sequencing. Each strain of S. pastorianus has lost species-specific portions of its genome and has undergone extensive recombination, producing chimeric chromosomes. We predicted 30 breakpoints that we confirmed at the single nucleotide level by designing species-specific primers that flank each breakpoint, and then sequencing the PCR product. These rearrangements are the result of recombination between areas of homology between the two subgenomes, rather than repetitive elements such as transposons or tRNAs. Interestingly, 28/30 S. cerevisiae-S. eubayanus recombination breakpoints are located within genic regions, generating chimeric genes. Furthermore we show evidence for the reuse of two breakpoints, located in HSP82 and KEM1, in strains of proposed independent origin.
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