ArticleThe Plant cell2014
N-glycosylation of effector proteins by an α-1,3-mannosyltransferase is required for the rice blast fungus to evade host innate immunity.
Article in The Plant cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers.
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Who cites it
89 citing papers in PubMed, 172 citations in OpenAlex.
- The glycosylated root-knot nematode effector Minc10750 suppresses plant immunity by destabilizing host chitinases.The New phytologist · 2026Article
- The oligosaccharyltransferase TaOST1B promotes viral infection by enhancement of RNA silencing suppression in wheat.Nature communications · 2026Article
- Integrative multi-omics identifies a glycosylation-based prognostic framework and nominates ALG3 for targeted therapy in bladder cancer.Discover oncology · 2026Article
- Histone Demethylase MoRph1 Regulates Fungal Development, Pathogenicity, and DNA Damage Repair inJournal of fungi (Basel, Switzerland) · 2026Article
- Lysine crotonylation enhances fungal effector activity and promotes invasion of host plants.Plant communications · 2026Article
- Targeting the EffectorFoods (Basel, Switzerland) · 2026Article
- Emerging roles of protein modifications in sexual reproduction and pathogenesis of filamentous fungi.Frontiers in microbiology · 2026Review
- Integrative perspectives on glycosylation networks in fungi and oomycetes.Journal of microbiology (Seoul, Korea) · 2025Review
- The Signal Peptidase MoSpc2 Orchestrates Fungal Development, Protein Secretion and Pathogenicity in Magnaporthe oryzae.Rice (New York, N.Y.) · 2025Article
- Functions ofJournal of fungi (Basel, Switzerland) · 2025Review
- Integrative glycomic analysis reveals the crucial role of protein glycosylation in fungal pathogenesis.PLoS pathogens · 2025Article
- N-Glycosylation-The Behind-the-Scenes 'Manipulative Hand' of Plant Pathogen Invasiveness.Molecular plant pathology · 2025Review
- Investigation of the mechanisms involved in the biocontrol activities of natural products from a marine soil bacterium against rice blast.Pest management science · 2025Article
- Live cell imaging of plant infection provides new insight into the biology of pathogenesis by the rice blast fungus Magnaporthe oryzae.Journal of microscopy · 2025Review
- Functional Analysis of KPvRxLR27, a Novel Plasmopara viticola Effector from a Korean Isolate, and Its Role in Hypersensitive Response.The plant pathology journal · 2025Article
- Individually redundant effectors are collectively required for bacterial pathogen virulence.The ISME journal · 2025Article
- Phosphite inhibitsFrontiers in microbiology · 2025Article
- Exploring the infection strategy ofFrontiers in plant science · 2025Article
- Article
- Pyricularia oryzae: Lab star and field scourge.Molecular plant pathology · 2024Review
29 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant pathogenic fungi deploy secreted effectors to suppress plant immunity responses. These effectors operate either in the apoplast or within host cells, so they are putatively glycosylated, but the posttranslational regulation of their activities has not been explored. In this study, the ASPARAGINE-LINKED GLYCOSYLATION3 (ALG3)-mediated N-glycosylation of the effector, Secreted LysM Protein1 (Slp1), was found to be essential for its activity in the rice blast fungus Magnaporthe oryzae. ALG3 encodes an α-1,3-mannosyltransferase for protein N-glycosylation. Deletion of ALG3 resulted in the arrest of secondary infection hyphae and a significant reduction in virulence. We observed that Δalg3 mutants induced massive production of reactive oxygen species in host cells, in a similar manner to Δslp1 mutants, which is a key factor responsible for arresting infection hyphae of the mutants. Slp1 sequesters chitin oligosaccharides to avoid their recognition by the rice (Oryza sativa) chitin elicitor binding protein CEBiP and the induction of innate immune responses, including reactive oxygen species production. We demonstrate that Slp1 has three N-glycosylation sites and that simultaneous Alg3-mediated N-glycosylation of each site is required to maintain protein stability and the chitin binding activity of Slp1, which are essential for its effector function. These results indicate that Alg3-mediated N-glycosylation of Slp1 is required to evade host innate immunity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.