ArticlePloS one2014
A flow cytometric analysis of the inhibition of platelet reactivity due to nitrite reduction by deoxygenated erythrocytes.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 32 citations in OpenAlex.
- Conjugated Linoleic Acid Modulates Clinical Responses to Oral Nitrite and Nitrate.Hypertension (Dallas, Tex. : 1979) · 2017Trial
- Green Tea Epigallocatechin 3-Gallate Reduced Platelet Aggregation and Improved Anticoagulant Proteins in Patients with Transfusion-Dependent β-Thalassemia: A Randomized Placebo-Controlled Clinical Trial.Foods (Basel, Switzerland) · 2024Article
- The chemical biology of dinitrogen trioxide.Redox biochemistry and chemistry · 2024Article
- Nitrite decreases sickle hemoglobin polymerization in vitro independently of methemoglobin formation.Toxicology and applied pharmacology · 2023Article
- Review
- Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation.Journal of visualized experiments : JoVE · 2019Article
- A mononuclear nonheme {FeNO}Chemical science · 2018Article
- Identification of a soluble guanylate cyclase in RBCs: preserved activity in patients with coronary artery disease.Redox biology · 2018Article
- Article
- Hypoxia impairs agonist-induced integrin αScientific reports · 2017Article
- Potential therapeutic action of nitrite in sickle cell disease.Redox biology · 2017Article
- Red Blood Cell Function and Dysfunction: Redox Regulation, Nitric Oxide Metabolism, Anemia.Antioxidants & redox signaling · 2017Review
- AltitudeOmics: Red Blood Cell Metabolic Adaptation to High Altitude Hypoxia.Journal of proteome research · 2016Article
- Can erythrocytes release biologically active NO?Cell communication and signaling : CCS · 2016Article
- Erythrocytes do not activate purified and platelet soluble guanylate cyclases even in conditions favourable for NO synthesis.Cell communication and signaling : CCS · 2016Article
- Globin X is a six-coordinate globin that reduces nitrite to nitric oxide in fish red blood cells.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Mechanisms of human erythrocytic bioactivation of nitrite.The Journal of biological chemistry · 2015Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Nitric oxide (NO), a small gas molecule, has long been known to be a potent inhibitor of platelet function but the physiological and pathological implications of platelet inhibition by NO have not been well clarified. We recently showed that the addition of nitrite to platelet-rich plasma in the presence of erythrocytes could inhibit platelet aggregation and this inhibitory effect of nitrite + erythrocytes was enhanced by deoxygenation of erythrocytes as measured by P-selectin expression and cGMP production. In order to study the nitrite effect on platelets at different oxygen levels, we used the flow cytometric assays to detect platelet membrane surface markers upon activation. The P-selectin and activated gpIIb/IIIa expression on platelet membranes in response to ADP, collagen and thrombin stimulation was measured at various hematocrit and oxygen levels. Nitrite (0.1 to 1.0 μM) significantly decreased the percentage of these surface markers on the platelet membrane at the hematocrit values above 23% and oxygen levels lower than 49 mmHg. The inhibitory effect of nitrite was augmented by increasing hematocrit values and decreasing oxygen saturation. C-PTIO (an NO scavenger) prevented the platelet inhibition by nitrite + erythrocytes whereas the inhibitors of NO synthase and xanthine oxidoreductase had no effect. These results support the proposal that circulating nitrite decreases platelet reactivity in the presence of partially deoxygenated erythrocytes through its reduction to NO, which may also explain certain differences between arterial and venous thrombosis and support directly the role of deoxyhemoglobin in this process. We believe that our flow cytometric assays offer a possibility to identify the individual molecular process involved in these effects.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.