ArticlePloS one2014
Detection and occurrence of plasmid-mediated AmpC in highly resistant gram-negative rods.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 43 citations in OpenAlex.
- Mapping the Thematic Structure and Evolution of AmpC β-Lactamase Research: A Bibliometric Analysis.Antibiotics (Basel, Switzerland) · 2026Article
- Frequency of CIT, EBC and DHA flocks (families) of AmpC beta-lactamases in clinical isolates of Klebsiella pneumoniae collected from hospitalized patients in North of Iran.Molecular biology reports · 2026Article
- Analyses of Extended-Spectrum-β-Lactamase, Metallo-β-Lactamase, and AmpC-β-Lactamase Producing Enterobacteriaceae from the Dairy Value Chain in India.Antibiotics (Basel, Switzerland) · 2023Article
- Detection of plasmid-mediated AmpC β-lactamases inIranian journal of microbiology · 2023Article
- Phenotypic and genotypic detection methods for antimicrobial resistance in ESKAPE pathogens (Review).Experimental and therapeutic medicine · 2022Review
- Antibiotic resistance in the invasive bacteria Escherichia coli.Central European journal of public health · 2022Article
- Systematic Review of Plasmid AmpC Type Resistances inMicroorganisms · 2022Article
- A novel air-dried multiplex high-resolution melt assay for the detection of extended-spectrum β-lactamase and carbapenemase genes.Journal of global antimicrobial resistance · 2021Article
- Occurrence, antimicrobial susceptibility, and pathogenic factors ofVeterinary world · 2021Article
- Detection of CMY-type beta-lactamases in Escherichia coli isolates from paediatric patients in a tertiary care hospital in Mexico.Antimicrobial resistance and infection control · 2020Article
- Prevalence of plasmid-mediated AmpC beta-lactamases in Enterobacteria isolated from urban and rural folks in Uganda.AAS open research · 2020Article
- A Primer on AmpC β-Lactamases: Necessary Knowledge for an Increasingly Multidrug-resistant World.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2019Review
- Phenotypic Characterization and Antibiotic Resistance Patterns of Extended-SpectrumThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2019Article
- Impact of InducibleAntimicrobial agents and chemotherapy · 2018Article
- Plasmids carrying DHA-1 β-lactamases.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2018Review
- Multiplex PCR scheme for variant plasmid mediated class C β-lactamase typing.Journal of clinical laboratory analysis · 2018Article
- Co-Occurrence of Plasmid-Mediated AmpC β-Lactamase Activity AmongThe open microbiology journal · 2017Article
- Dissemination of Extended-Spectrum β-Lactamase- and AmpC β-Lactamase-Producing Escherichia coli within the Food Distribution System of Ho Chi Minh City, Vietnam.BioMed research international · 2016Article
- Plasmid-mediated AmpC: prevalence in community-acquired isolates in Amsterdam, the Netherlands, and risk factors for carriage.PloS one · 2015Article
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Authors and funding
9 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesThe aim of this study was to compare the current screening methods and to evaluate confirmation tests for phenotypic plasmidal AmpC (pAmpC) detection.
methodsFor this evaluation we used 503 Enterobacteriaceae from 18 Dutch hospitals and 21 isolates previously confirmed to be pAmpC positive. All isolates were divided into three groups: isolates with 1) reduced susceptibility to ceftazidime and/or cefotaxime; 2) reduced susceptibility to cefoxitin; 3) reduced susceptibility to ceftazidime and/or cefotaxime combined with reduced susceptibility to cefoxitin. Two disk-based tests, with cloxacillin or boronic acid as inhibitor, and Etest with cefotetan-cefotetan/cloxacillin were used for phenotypic AmpC confirmation. Finally, presence of pAmpC genes was tested by multiplex and singleplex PCR.
resultsWe identified 13 pAmpC producing Enterobacteriaceae isolates among the 503 isolates (2.6%): 9 CMY-2, 3 DHA-1 and 1 ACC-1 type in E. coli isolates. The sensitivity and specificity of reduced susceptibility to ceftazidime and/or cefotaxime in combination with cefoxitin was 97% (33/34) and 90% (289/322) respectively. The disk-based test with cloxacillin showed the best performance as phenotypic confirmation method for AmpC production.
conclusionsFor routine phenotypic detection of pAmpC the screening for reduced susceptibility to third generation cephalosporins combined with reduced susceptibility to cefoxitin is recommended. Confirmation via a combination disk diffusion test using cloxacillin is the best phenotypic option. The prevalence found is worrisome, since, due to their plasmidal location, pAmpC genes may spread further and increase in prevalence.
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