ArticleJournal of immunological methods2014
Limitations of Foxp3(+) Treg depletion following viral infection in DEREG mice.
Article in Journal of immunological methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
34 citing papers in PubMed, 46 citations in OpenAlex.
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- Effects of Regulatory T Cell Depletion in BALB/c Mice Infected with Low Doses ofPathogens (Basel, Switzerland) · 2023Article
- Therapeutic Effect of Anti-CD52 Monoclonal Antibody in Multiple Sclerosis and Its Animal Models Is Mediated via T Regulatory Cells.Journal of immunology (Baltimore, Md. : 1950) · 2022Article
- Adoptive therapy with amyloid-β specific regulatory T cells alleviates Alzheimer's disease.Theranostics · 2022Article
- Effects of Recombinant IL-35-BCG on Treg/Th17 Cell Imbalance and Inflammatory Response in Asthmatic Newborn Mice Induced by RSV.Inflammation · 2021Article
- CD85k Contributes to Regulatory T Cell Function in Chronic Viral Infections.International journal of molecular sciences · 2020Article
- Bone marrow Tregs mediate stromal cell function and support hematopoiesis via IL-10.JCI insight · 2020Article
- Regulatory T Cells Contribute to Resistance against Lyme Arthritis.Infection and immunity · 2020Article
- Intein-mediated cytoplasmic reconstitution of a split toxin enables selective cell ablation in mixed populations and tumor xenografts.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Versatile cell ablation tools and their applications to study loss of cell functions.Cellular and molecular life sciences : CMLS · 2019Review
- Prophylactic Effects of Bee Venom Phospholipase A2 in Lipopolysaccharide-Induced Pregnancy Loss.Toxins · 2019Article
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- Foxp3Infection and immunity · 2018Article
- CD4Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- Principles of Immunotherapy: Implications for Treatment Strategies in Cancer and Infectious Diseases.Frontiers in microbiology · 2018Review
- Depletion of FoxP3Immunity, inflammation and disease · 2017Article
- Regulatory T cells promote myelin regeneration in the central nervous system.Nature neuroscience · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Regulatory T cells (Tregs) play a critical role in maintaining tissue homeostasis and preventing the development of immunopathology. Depletion of REGulatory T cell (DEREG) mice express a diphtheria toxin receptor (DTR)-eGFP transgene under the control of the Foxp3 promoter allowing for Treg depletion following diphtheria toxin (DT) administration. DEREG mice have been utilized to investigate the role of Tregs in a wide range of disease settings. Administration of DT to naïve DEREG mice resulted in the rapid depletion of Foxp3(+) Tregs from the peripheral blood. However, by day 4 post-DT administration, a GFP(-) Foxp3(+) Treg population emerged that lacked expression of the DTR transgene and was resistant to further depletion by additional DT treatment. We further evaluated the impact of Treg depletion during both acute and chronic viral infections. Similar to naïve mice, Treg numbers rapidly rebounded during an inflammatory setting following an acute viral infection. DT treatment of both wild-type (WT) and DEREG mice following both acute and chronic viral infections induced exacerbated disease as compared to PBS-treated controls. Furthermore, following a chronic systemic viral infection, DT treatment resulted in nearly 100% mortality in both WT and DEREG mice while the PBS-treated controls survived. Our results demonstrate that Treg depletion in DEREG mice is transient and that DT administration can have adverse effects during virus-induced inflammation and highlights the critical need to include DT-treated WT mice when using DTR models to control for DT-mediated toxicity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.