Evidence map›Paper›PMID 24639751›Full record

ArticleIranian journal of reproductive medicine2013

Characterization of mesenchymal stem cells from human dental pulp, preapical follicle and periodontal ligament.

Ali Reza Navabazam, Fatemeh Sadeghian Nodoshan, Mohammad Hasan Sheikhha, Sayyed Mohsen Miresmaeili, Mehrdad Soleimani, Farzaneh Fesahat

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Article in Iranian journal of reproductive medicine, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.

0numbers the graph read from it
0cells of the map it votes in
26citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

26 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Ali Reza NavabazamDepartment of Oral and Maxillo-facial Surgery, Dental School, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Fatemeh Sadeghian NodoshanResearch and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Mohammad Hasan SheikhhaResearch and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Sayyed Mohsen MiresmaeiliYazd Academic Center of Education, Culture and Research Higher Education Institute, Yazd, Iran.
Mehrdad SoleimaniResearch and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Farzaneh FesahatResearch and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundHuman dental stem cells have high proliferative potential for self-renewal that is important to the regenerative capacity of the tissue. Objective : The aim was to isolate human dental pulp stem cells (DPSC), periodontal ligament stem cells (PDLSC) and periapical follicle stem cells (PAFSC) for their potential role in tissue regeneration. METHODS & MATERIALS: In this experimental study, the postnatal stem cells were isolated from dental pulp, preapical follicle and periodontal ligament .The cells were stained for different stem cell markers by immunocytochemistry. To investigate the mesenchymal nature of cells, differentiation potential along osteoblastic and adipogenic lineages and gene expression profile were performed. For proliferation potential assay, Brdu staining and growth curve tests were performed. Finally, all three cell types were compared together regarding their proliferation, differentiation and displaying phenotype.

resultsThe isolated cell populations have similar fibroblastic like morphology and expressed all examined cell surface molecule markers. These cells were capable of differentiating into osteocyte with different capability and adipocyte with the same rate. PAFSCs showed more significant proliferation rate than others. Reverse transcriptase PCR (RT-PCR) for nanog, oct4, Alkaline phosphatase (ALP) and glyceraldehydes-3-phosphate dehydrogenease (GADPH) as control gene showed strong positive expression of these genes in all three isolated cell types.

conclusionPDLSCs, DPSCs and PAFSCs exist in various tissues of the teeth and can use as a source of mesenchymal stem cells for developing bioengineered organs and also in craniomaxillofacial reconstruction with varying efficiency in differentiation and proliferation.

Indexed as

Dental pulpMesenchymal stem cellPeriodontal ligament stem cellsPreapical follicle

Identifiers

PMID24639751
PMCPMC3943215

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.