ArticleMuscle & nerve2014
Improved immunoblotting methods provide critical insights into phenotypic differences between two murine dysferlinopathy models.
Article in Muscle & nerve, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed, 6 citations in OpenAlex.
- Article
- Pseudouridine synthase 1 deficient mice, a model for Mitochondrial Myopathy with Sideroblastic Anemia, exhibit muscle morphology and physiology alterations.Scientific reports · 2016Article
- Myofiber damage precedes macrophage infiltration after in vivo injury in dysferlin-deficient A/J mouse skeletal muscle.The American journal of pathology · 2015Article
- Calpain cleavage within dysferlin exon 40a releases a synaptotagmin-like module for membrane repair.Molecular biology of the cell · 2014Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
introductionWe adopted a proteomics-based approach to gain insights into phenotypic differences between A/J and B10.SJL murine dysferlinopathy models.
methodsWe optimized immunoblotting of dysferlin by preparing homogenates of the tibialis anterior (TA) muscle under several different conditions. We compared TA muscles of control, A/J, and B10.SJL mice for levels of dysferlin; dysferlin's partners MG53, annexin-A2, and caveolin-3; and the endoplasmic reticulum (ER) stress marker CHOP. We performed immunoelectron microscopy on control rat TA muscle to determine the precise location of dysferlin.
resultsRIPA (radioimmunoprecipitation assay) buffer and sonication improves immunoblotting of dysferlin. The ER stress marker CHOP is elevated in A/J muscle. Dysferlin is localized mostly to membranes close to the Z-disk that have been reported to be part of the Golgi, ER, and sarcoplasmic reticulum (SR) networks.
conclusionsER stress might underlie phenotypic differences between A/J and B10.SJL mice and play a role in human dysferlinopathies.
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