ArticleThe Journal of general physiology2014
Sarcomere length nanometry in rat neonatal cardiomyocytes expressed with α-actinin-AcGFP in Z discs.
Article in The Journal of general physiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 51 citations in OpenAlex.
- Open-source bioreactor delivers electrical and perfusion stimulation supporting 3D cardiac engineered tissue maturation.Bioengineering & translational medicine · 2026Article
- Structured neighboring-sarcomere switching is coupled to variable-reach internal length redistribution during hyperthermal sarcomeric oscillations in living cardiomyocytes.Biophysics and physicobiology · 2026Article
- Asynchronous movement of sarcomeres in myocardium under living conditions: role of titin.Frontiers in physiology · 2024Article
- Observation of sarcomere chaos induced by changes in calcium concentration in cardiomyocytes.Biophysics and physicobiology · 2024Article
- Impact of stretch on sarcomere length variability in isolated fully relaxed rat cardiac myocytes.Pflugers Archiv : European journal of physiology · 2023Article
- Comparing the effects of chemical CaJournal of molecular and cellular cardiology · 2023Article
- Impact of stretch on sarcomere length variability in isolated fully relaxed rat cardiac myocytes.Research square · 2023Article
- Cardiomyocyte sarcomere length variability: Membrane fluorescence versus second harmonic generation myosin imaging.The Journal of general physiology · 2023Article
- Editorial: Recent Advances on Myocardium Physiology, Volume II.Frontiers in physiology · 2023Article
- Real-time scanning electron microscopy of unfixed tissue in the solution using a deformable and electron-transmissive film.Microscopy (Oxford, England) · 2022Article
- Does the Hyperthermal Sarcomeric Oscillations Manifested by Body Temperature Support the Periodic Ventricular Dilation With Each Heartbeat?Frontiers in physiology · 2022Article
- Contractile State Dependent Sarcomere Length Variability in Isolated Guinea-Pig Cardiomyocytes.Frontiers in physiology · 2022Article
- Synchrony of sarcomeric movement regulates left ventricular pump function in the in vivo beating mouse heart.The Journal of general physiology · 2021Article
- Effects of high-pressure treatment on the structure and function of myofibrils.Biophysics and physicobiology · 2021Article
- Mechanism of contraction rhythm homeostasis for hyperthermal sarcomeric oscillations of neonatal cardiomyocytes.Scientific reports · 2020Article
- Single-cell temperature mapping with fluorescent thermometer nanosheets.The Journal of general physiology · 2020Article
- Effect of myofibril passive elastic properties on the mechanical communication between motor proteins on adjacent sarcomeres.Scientific reports · 2019Article
- SarcTrack.Circulation research · 2019Article
- Optimization of Fluorescent Labeling forBioMed research international · 2018Article
- In vitro and in vivo analysis of visible light crosslinkable gelatin methacryloyl (GelMA) hydrogels.Biomaterials science · 2017Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nanometry is widely used in biological sciences to analyze the movement of molecules or molecular assemblies in cells and in vivo. In cardiac muscle, a change in sarcomere length (SL) by a mere ∼100 nm causes a substantial change in contractility, indicating the need for the simultaneous measurement of SL and intracellular Ca(2+) concentration ([Ca(2+)]i) in cardiomyocytes at high spatial and temporal resolution. To accurately analyze the motion of individual sarcomeres with nanometer precision during excitation-contraction coupling, we applied nanometry techniques to primary-cultured rat neonatal cardiomyocytes. First, we developed an experimental system for simultaneous nanoscale analysis of single sarcomere dynamics and [Ca(2+)]i changes via the expression of AcGFP in Z discs. We found that the averaging of the lengths of sarcomeres along the myocyte, a method generally used in today's myocardial research, caused marked underestimation of sarcomere lengthening speed because of the superpositioning of different timings for lengthening between sequentially connected sarcomeres. Then, we found that after treatment with ionomycin, neonatal myocytes exhibited spontaneous sarcomeric oscillations (cell-SPOCs) at partial activation with blockage of sarcoplasmic reticulum functions, and the waveform properties were indistinguishable from those obtained in electric field stimulation. The myosin activator omecamtiv mecarbil markedly enhanced Z-disc displacement during cell-SPOC. Finally, we interpreted the present experimental findings in the framework of our mathematical model of SPOCs. The present experimental system has a broad range of application possibilities for unveiling single sarcomere dynamics during excitation-contraction coupling in cardiomyocytes under various settings.
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