ArticleJournal of visualized experiments : JoVE2014
Juxtasomal biocytin labeling to study the structure-function relationship of individual cortical neurons.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Thalamus enables active dendritic coupling of inputs arriving at different cortical layers.Nature communications · 2025Article
- Technical report: surgical preparation of human brain tissue for clinical and basic research.Acta neurochirurgica · 2023Article
- Different subtypes of motor cortex pyramidal tract neurons projects to red and pontine nuclei.Frontiers in cellular neuroscience · 2022Article
- Article
- Functional Architecture and Encoding of Tactile Sensorimotor Behavior in Rat Posterior Parietal Cortex.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Assessing the Impact of Single-Cell Stimulation on Local Networks in Rat Barrel Cortex-A Feasibility Study.International journal of molecular sciences · 2019Article
- Activation of Corticothalamic Layer 6 Cells Decreases Angular Tuning in Mouse Barrel Cortex.Frontiers in neural circuits · 2019Article
- Morphological characterization of HVC projection neurons in the zebra finch (Taeniopygia guttata).The Journal of comparative neurology · 2018Article
- Neural Representation of Motor Output, Context and Behavioral Adaptation in Rat Medial Prefrontal Cortex During Learned Behavior.Frontiers in neural circuits · 2018Article
- Relationships between structure, in vivo function and long-range axonal target of cortical pyramidal tract neurons.Nature communications · 2017Article
- Cell Type-Specific Structural Organization of the Six Layers in Rat Barrel Cortex.Frontiers in neuroanatomy · 2017Review
- Beyond Columnar Organization: Cell Type- and Target Layer-Specific Principles of Horizontal Axon Projection Patterns in Rat Vibrissal Cortex.Cerebral cortex (New York, N.Y. : 1991) · 2015Article
- Generation of dense statistical connectomes from sparse morphological data.Frontiers in neuroanatomy · 2014Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cerebral cortex is characterized by multiple layers and many distinct cell-types that together as a network are responsible for many higher cognitive functions including decision making, sensory-guided behavior or memory. To understand how such intricate neuronal networks perform such tasks, a crucial step is to determine the function (or electrical activity) of individual cell types within the network, preferentially when the animal is performing a relevant cognitive task. Additionally, it is equally important to determine the anatomical structure of the network and the morphological architecture of the individual neurons to allow reverse engineering the cortical network. Technical breakthroughs available today allow recording cellular activity in awake, behaving animals with the valuable option of post hoc identifying the recorded neurons. Here, we demonstrate the juxtasomal biocytin labeling technique, which involves recording action potential spiking in the extracellular (or loose-patch) configuration using conventional patch pipettes. The juxtasomal recording configuration is relatively stable and applicable across behavioral conditions, including anesthetized, sedated, awake head-fixed, and even in the freely moving animal. Thus, this method allows linking cell-type specific action potential spiking during animal behavior to reconstruction of the individual neurons and ultimately, the entire cortical microcircuit. In this video manuscript, we show how individual neurons in the juxtasomal configuration can be labeled with biocytin in the urethane-anaesthetized rat for post hoc identification and morphological reconstruction.
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