ArticlePloS one2014
Long term expansion of bone marrow-derived hMSCs on novel synthetic microcarriers in xeno-free, defined conditions.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed.
- Process intensification of cultivated meat production through microcarrier addition strategy optimisation.Scientific reports · 2025Article
- Biomaterials for Cell Manufacturing.ACS macro letters · 2024Article
- Mesenchymal and induced pluripotent stem cell-based therapeutics: a comparison.Applied microbiology and biotechnology · 2023Review
- Development of a novel feeding regime for large scale production of human umbilical cord mesenchymal stem/stromal cells.Cytotechnology · 2022Article
- Long term expansion profile of mesenchymal stromal cells at protein nanosheet-stabilised bioemulsions for next generation cell culture microcarriers.Materials today. Bio · 2021Article
- Facile bead-to-bead cell-transfer method for serial subculture and large-scale expansion of human mesenchymal stem cells in bioreactors.Stem cells translational medicine · 2021Article
- Micro computed tomography with and without contrast enhancement for the characterization of microcarriers in dry and wet state.Scientific reports · 2021Article
- Influence of Microenvironment on Mesenchymal Stem Cell Therapeutic Potency: From Planar Culture to Microcarriers.Frontiers in bioengineering and biotechnology · 2020Review
- Microcarriers for Upscaling Cultured Meat Production.Frontiers in nutrition · 2020Review
- Large-Scale Expansion of Human Mesenchymal Stem Cells.Stem cells international · 2020Review
- Modular Orthopaedic Tissue Engineering With Implantable Microcarriers and Canine Adipose-Derived Mesenchymal Stromal Cells.Frontiers in bioengineering and biotechnology · 2020Article
- Preparing for cell culture scale-out: establishing parity of bioreactor- and flask-expanded mesenchymal stromal cell cultures.Journal of translational medicine · 2019Article
- The Evolution of Polystyrene as a Cell Culture Material.Tissue engineering. Part B, Reviews · 2018Review
- Adhesion, proliferation and osteogenic differentiation of mesenchymal stem cells in 3D printed poly-ε-caprolactone/hydroxyapatite scaffolds combined with bone marrow clots.Molecular medicine reports · 2017Article
- Process development of human multipotent stromal cell microcarrier culture using an automated high-throughput microbioreactor.Biotechnology and bioengineering · 2017Article
- Mesenchymal stromal cell-based therapy: Regulatory and translational aspects in gastroenterology.World journal of gastroenterology · 2016Review
- Endothelin-1 supports clonal derivation and expansion of cardiovascular progenitors derived from human embryonic stem cells.Nature communications · 2016Article
- Bioprocessing strategies for the large-scale production of human mesenchymal stem cells: a review.Stem cell research & therapy · 2015Review
- Robust Expansion of Human Pluripotent Stem Cells: Integration of Bioprocess Design With Transcriptomic and Metabolomic Characterization.Stem cells translational medicine · 2015Article
- Defined culture of human embryonic stem cells and xeno-free derivation of retinal pigmented epithelial cells on a novel, synthetic substrate.Stem cells translational medicine · 2015Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Human mesenchymal stem cells (hMSCs) present an attractive target for cell therapy given their wide availability, immunomodulatory properties, and multipotent nature for differentiation into chondrocytes, osteocytes, and adipocytes. With the progression of hMSC clinical studies, there is an increasing demand for development of technologies that enable efficient cell scale-up into clinically relevant quantities. Commercial scale manufacturing of hMSCs will require a large surface area which is not cost effective with available two-dimensional culture vessels. Recent studies showed that microcarriers provide a three-dimensional culture environment suitable for hMSC expansion. Traditionally, biological coatings and/or serum-containing medium are required to facilitate hMSC attachment and expansion in dynamic conditions. These limitations may hinder the use of microcarriers as a scale-up technology for hMSC therapeutics, where cell products, and therefore patient safety, are more controlled with the use of xeno-free, defined culture conditions. Here we report the long term culture of hMSCs on novel synthetic Synthemax II microcarriers in two different xeno-free media. Cells were maintained over 40 days on sterile, ready-to-use microcarriers in spinner flasks with programmed agitation. hMSC expansion was obtained by addition of fresh beads without the need for enzymatic dissociation. We achieved a cumulative cell expansion of >10,000 fold, and cells retained normal hMSC phenotype, karyotype, and tri-lineage differentiation potential. To our knowledge, this report is the first example of long term culture of hMSCs on synthetic microcarriers in xeno-free, defined conditions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.