ArticleJournal of visualized experiments : JoVE2014
Dissection of Xenopus laevis neural crest for in vitro explant culture or in vivo transplantation.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- A time-resolved single-cell roadmap of the logic driving anterior neural crest diversification from neural border to migration stages.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Live Imaging of Cytoskeletal Dynamics in EmbryonicCold Spring Harbor protocols · 2021Article
- The transcription factor Hypermethylated in Cancer 1 (Hic1) regulates neural crest migration via interaction with Wnt signaling.Developmental biology · 2020Article
- Wolf-Hirschhorn Syndrome-Associated Genes Are Enriched in Motile Neural Crest Cells and Affect Craniofacial Development inFrontiers in physiology · 2019Article
- The Many Faces ofFrontiers in physiology · 2019Article
- TBC1d24-ephrinB2 interaction regulates contact inhibition of locomotion in neural crest cell migration.Nature communications · 2018Article
- Redistribution of Adhesive Forces through Src/FAK Drives Contact Inhibition of Locomotion in Neural Crest.Developmental cell · 2018Article
- Glycogen synthase kinase 3 controls migration of the neural crest lineage in mouse and Xenopus.Nature communications · 2018Article
- A molecular atlas of the developing ectoderm defines neural, neural crest, placode, and nonneural progenitor identity in vertebrates.PLoS biology · 2017Article
- Modeling human craniofacial disorders inCurrent pathobiology reports · 2017Article
- Exploring the developmental mechanisms underlying Wolf-Hirschhorn Syndrome: Evidence for defects in neural crest cell migration.Developmental biology · 2016Review
- Frogs as integrative models for understanding digestive organ development and evolution.Seminars in cell & developmental biology · 2016Review
- Stem cell-like Xenopus Embryonic Explants to Study Early Neural Developmental Features In Vitro and In Vivo.Journal of visualized experiments : JoVE · 2016Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The neural crest (NC) is a transient dorsal neural tube cell population that undergoes an epithelium-to-mesenchyme transition (EMT) at the end of neurulation, migrates extensively towards various organs, and differentiates into many types of derivatives (neurons, glia, cartilage and bone, pigmented and endocrine cells). In this protocol, we describe how to dissect the premigratory cranial NC from Xenopus laevis embryos, in order to study NC development in vivo and in vitro. The frog model offers many advantages to study early development; abundant batches are available, embryos develop rapidly, in vivo gain and loss of function strategies allow manipulation of gene expression prior to NC dissection in donor and/or host embryos. The NC explants can be plated on fibronectin and used for in vitro studies. They can be cultured for several days in a serum-free defined medium. We also describe how to graft NC explants back into host embryos for studying NC migration and differentiation in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.