ArticlePloS one2014
Expression analysis of SOX14 during retinoic acid induced neural differentiation of embryonal carcinoma cells and assessment of the effect of its ectopic expression on SOXB members in HeLa cells.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- Overexpression of miR-219 as a potential therapeutic strategy of glioblastoma cells in vitro.Scientific reports · 2026Article
- The Role of SOX2 and SOX9 Transcription Factors in the Reactivation-Related Functional Properties of NT2/D1-Derived Astrocytes.Biomedicines · 2024Article
- The Role of SOX Transcription Factors in Ageing and Age-Related Diseases.International journal of molecular sciences · 2023Review
- Reactive and Senescent Astroglial Phenotypes as Hallmarks of Brain Pathologies.International journal of molecular sciences · 2022Review
- Reference Module-Based Analysis of Ovarian Cancer Transcriptome Identifies Important Modules and Potential Drugs.Biochemical genetics · 2022Article
- SOX Transcription Factors as Important Regulators of Neuronal and Glial Differentiation During Nervous System Development and Adult Neurogenesis.Frontiers in molecular neuroscience · 2021Review
- Insights into platinum-induced peripheral neuropathy-current perspective.Neural regeneration research · 2020Review
- Neuroprotective Role of Selected Antioxidant Agents in Preventing Cisplatin-Induced Damage of Human Neurons In Vitro.Cellular and molecular neurobiology · 2019Article
- SOX3 can promote the malignant behavior of glioblastoma cells.Cellular oncology (Dordrecht, Netherlands) · 2019Article
- Duplication and expression of Sox genes in spiders.BMC evolutionary biology · 2018Article
- Directed differentiation of mouse P19 embryonal carcinoma cells to neural cells in a serum- and retinoic acid-free culture medium.In vitro cellular & developmental biology. Animal · 2018Article
- Sequential Role of SOXB2 Factors in GABAergic Neuron Specification of the Dorsal Midbrain.Frontiers in molecular neuroscience · 2018Article
- SOX14 activates the p53 signaling pathway and induces apoptosis in a cervical carcinoma cell line.PloS one · 2017Article
- Epigenetic regulation of human SOX3 gene expression during early phases of neural differentiation of NT2/D1 cells.PloS one · 2017Article
- Gene Expression Profiling Reveals a Novel Regulatory Role for Sox21 Protein in Mouse Trophoblast Stem Cell Differentiation.The Journal of biological chemistry · 2015Article
- Crosstalk between SOXB1 proteins and WNT/β-catenin signaling in NT2/D1 cells.Histochemistry and cell biology · 2015Article
- Enhanced SOX2 expression in retinoblastoma tissues and peripheral blood is associated with the clinicopathological characteristics of the disease.Oncology letters · 2015Article
- Article
- SOX14 promotes proliferation and invasion of cervical cancer cells through Wnt/β-catenin pathway.International journal of clinical and experimental pathology · 2015Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
SOX14 is a member of the SOXB2 subgroup of transcription factors implicated in neural development. Although the first SOX14 gene in vertebrates was cloned and characterized more than a decade ago and its expression profile during development was revealed in various animal model systems, the role of this gene during neural development is largely unknown. In the present study we analyzed the expression of SOX14 in human NT2/D1 and mouse P19 pluripotent embryonal carcinoma cells. We demonstrated that it is expressed in both cell lines and upregulated during retinoic acid induced neural differentiation. We showed that SOX14 was expressed in both neuronal and non-neuronal differentiated derivatives, as revealed by immunocytochemistry. Since it was previously proposed that increased SOXB2 proteins level interfere with the activity of SOXB1 counteracting partners, we compared expression patterns of SOXB members during retinoic acid induction of embryonal carcinoma cells. We revealed that upregulation of SOX14 expression is accompanied by alterations in the expression patterns of SOXB1 members. In order to analyze the potential cross-talk between them, we generated SOX14 expression construct. The ectopic expression of SOX14 was demonstrated at the mRNA level in NT2/D1, P19 and HeLa cells, while an increased level of SOX14 protein was detected in HeLa cells only. By transient transfection experiments in HeLa cells we showed for the first time that ectopic expression of SOX14 repressed SOX1 expression, whereas no significant effect on SOX2, SOX3 and SOX21 was observed. Data presented here provide an insight into SOX14 expression during in vitro neural differentiation of embryonal carcinoma cells and demonstrate the effect of its ectopic expression on protein levels of SOXB members in HeLa cells. Obtained results contribute to better understanding the role of one of the most conserved SOX proteins.
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