ArticleNucleus (Austin, Tex.)
Single cell correlation fractal dimension of chromatin: a framework to interpret 3D single molecule super-resolution.
Article in Nucleus (Austin, Tex.). The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Article
- Binding memory of liquid molecules.Nature communications · 2025Article
- Mapping the nuclear landscape with multiplexed super-resolution fluorescence microscopy.Nature communications · 2025Article
- Correlative single molecule lattice light sheet imaging reveals the dynamic relationship between nucleosomes and the local chromatin environment.Nature communications · 2024Article
- A proposed unified interphase nucleus chromosome structure: Preliminary preponderance of evidence.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- A proposed unified mitotic chromosome architecture.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- The needle and the haystack: single molecule tracking to probe the transcription factor search in eukaryotes.Biochemical Society transactions · 2021Review
- The hierarchical packing of euchromatin domains can be described as multiplicative cascades.PLoS computational biology · 2021Article
- Complexus cordisArchivos de cardiologia de Mexico · 2020Article
- Protein motion in the nucleus: from anomalous diffusion to weak interactions.Biochemical Society transactions · 2018Review
- Non-specific interactions govern cytosolic diffusion of nanosized objects in mammalian cells.Nature materials · 2018Article
- Single-molecule fluorescence microscopy review: shedding new light on old problems.Bioscience reports · 2017Review
- Multi-scale tracking reveals scale-dependent chromatin dynamics after DNA damage.Molecular biology of the cell · 2017Article
- Volume Transitions of Isolated Cell Nuclei Induced by Rapid Temperature Increase.Biophysical journal · 2017Article
- PCNA appears in two populations of slow and fast diffusion with a constant ratio throughout S-phase in replicating mammalian cells.Scientific reports · 2016Article
- Genetically targeted fluorogenic macromolecules for subcellular imaging and cellular perturbation.Biomaterials · 2015Article
- Probing the target search of DNA-binding proteins in mammalian cells using TetR as model searcher.Nature communications · 2015Article
- Abundance and distribution of RNA polymerase II in Arabidopsis interphase nuclei.Journal of experimental botany · 2015Article
- Imaging Transcription: Past, Present, and Future.Cold Spring Harbor symposia on quantitative biology · 2015Review
- Inside single cells: quantitative analysis with advanced optics and nanomaterials.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnologyReview
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6 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Chromatin is a major nuclear component, and it is an active matter of debate to understand its different levels of spatial organization, as well as its implication in gene regulation. Measurements of nuclear chromatin compaction were recently used to understand how DNA is folded inside the nucleus and to detect cellular dysfunctions such as cancer. Super-resolution imaging opens new possibilities to measure chromatin organization in situ. Here, we performed a direct measure of chromatin compaction at the single cell level. We used histone H2B, one of the 4 core histone proteins forming the nucleosome, as a chromatin density marker. Using photoactivation localization microscopy (PALM) and adaptive optics, we measured the three-dimensional distribution of H2B with nanometric resolution. We computed the distribution of distances between every two points of the chromatin structure, namely the Ripley K(r) distribution. We found that the K(r) distribution of H2B followed a power law, leading to a precise measurement of the correlation fractal dimension of chromatin of 2.7. Moreover, using photoactivable GFP fused to H2B, we observed dynamic evolution of chromatin sub-regions compaction. As a result, the correlation fractal dimension of chromatin reported here can be interpreted as a dynamically maintained non-equilibrium state.
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