ArticleActa crystallographica. Section F, Structural biology communications2014
Approaches to automated protein crystal harvesting.
Article in Acta crystallographica. Section F, Structural biology communications, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed.
- Towards routine accurate electron-density studies of biological macromolecules.Acta crystallographica. Section D, Structural biology · 2026Article
- AXIS: a Lab-in-the-Loop machine learning approach for automated detection of macromolecular crystals.IUCrJ · 2026Article
- Crystallographic Ensembles Reveal the Structural Basis of Binding Entropy in SARS-CoV2 Macrodomain.bioRxiv : the preprint server for biology · 2025Article
- Purification, characterization, and preliminary serial crystallography diffraction advances structure determination of full-length human particulate guanylyl cyclase A receptor.Scientific reports · 2022Article
- Facilitated crystal handling using a simple device for evaporation reduction in microtiter plates.Journal of applied crystallography · 2021Article
- The low-cost Shifter microscope stage transforms the speed and robustness of protein crystal harvesting.Acta crystallographica. Section D, Structural biology · 2021Article
- Protein X-ray Crystallography and Drug Discovery.Molecules (Basel, Switzerland) · 2020Review
- A comparison of gas stream cooling and plunge cooling of macromolecular crystals.Journal of applied crystallography · 2019Article
- On-chip crystallization for serial crystallography experiments and on-chip ligand-binding studies.IUCrJ · 2019Article
- Using sound pulses to solve the crystal-harvesting bottleneck.Acta crystallographica. Section D, Structural biology · 2018Article
- X-ray transparent microfluidic platforms for membrane protein crystallization with microseeds.Lab on a chip · 2018Article
- Towards a compact and precise sample holder for macromolecular crystallography.Acta crystallographica. Section D, Structural biology · 2017Article
- Successful completion of a semi-automated enzyme-free cloning method.Journal of structural and functional genomics · 2016Article
- Automated harvesting and processing of protein crystals through laser photoablation.Acta crystallographica. Section D, Structural biology · 2016Article
- Fully automatic characterization and data collection from crystals of biological macromolecules.Acta crystallographica. Section D, Biological crystallography · 2015Article
- Precise Manipulation and Patterning of Protein Crystals for Macromolecular Crystallography Using Surface Acoustic Waves.Small (Weinheim an der Bergstrasse, Germany) · 2015Article
- Practical macromolecular cryocrystallography.Acta crystallographica. Section F, Structural biology communications · 2015Review
- Acoustic transfer of protein crystals from agarose pedestals to micromeshes for high-throughput screening.Acta crystallographica. Section D, Biological crystallography · 2015Article
- Improved reproducibility of unit-cell parameters in macromolecular cryocrystallography by limiting dehydration during crystal mounting.Acta crystallographica. Section D, Biological crystallography · 2014Article
- Automation in biological crystallization.Acta crystallographica. Section F, Structural biology communications · 2014Article
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Abstract
The harvesting of protein crystals is almost always a necessary step in the determination of a protein structure using X-ray crystallographic techniques. However, protein crystals are usually fragile and susceptible to damage during the harvesting process. For this reason, protein crystal harvesting is the single step that remains entirely dependent on skilled human intervention. Automation has been implemented in the majority of other stages of the structure-determination pipeline, including cloning, expression, purification, crystallization and data collection. The gap in automation between crystallization and data collection results in a bottleneck in throughput and presents unfortunate opportunities for crystal damage. Several automated protein crystal harvesting systems have been developed, including systems utilizing microcapillaries, microtools, microgrippers, acoustic droplet ejection and optical traps. However, these systems have yet to be commonly deployed in the majority of crystallography laboratories owing to a variety of technical and cost-related issues. Automation of protein crystal harvesting remains essential for harnessing the full benefits of fourth-generation synchrotrons, free-electron lasers and microfocus beamlines. Furthermore, automation of protein crystal harvesting offers several benefits when compared with traditional manual approaches, including the ability to harvest microcrystals, improved flash-cooling procedures and increased throughput.
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