ArticleJournal of visualized experiments : JoVE2014
Mouse embryonic development in a serum-free whole embryo culture system.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed.
- Depth-Resolved Attenuation Coefficient Quantification During Murine Embryonic Brain Development.Journal of biophotonics · 2025Article
- Multimodal imaging system combining optical coherence tomography and Brillouin microscopy for neural tube imaging.Optics letters · 2022Article
- Neuroblastoma differentiation in vivo excludes cranial tumors.Developmental cell · 2021Article
- Programmed Cell Senescence in the Mouse Developing Spinal Cord and Notochord.Frontiers in cell and developmental biology · 2021Article
- Mouse whole embryo culture: Evaluating the requirement for rat serum as culture medium.Birth defects research · 2019Article
- Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone.Journal of visualized experiments : JoVE · 2019Article
- Toward Microsurgical Correction of Cleft Lip Ex Utero through Restoration of Craniofacial Developmental Programs.Plastic and reconstructive surgery · 2017Article
- Applying evolutionary genetics to developmental toxicology and risk assessment.Reproductive toxicology (Elmsford, N.Y.) · 2017Review
- Using Ex Vivo Upright Droplet Cultures of Whole Fetal Organs to Study Developmental Processes during Mouse Organogenesis.Journal of visualized experiments : JoVE · 2015Article
- Analysis of cardiomyocyte development using immunofluorescence in embryonic mouse heart.Journal of visualized experiments : JoVE · 2015Article
- Contrast imaging in mouse embryos using high-frequency ultrasound.Journal of visualized experiments : JoVE · 2015Article
- Preparation of rat serum suitable for mammalian whole embryo culture.Journal of visualized experiments : JoVE · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mid-gestation stage mouse embryos were cultured utilizing a serum-free culture medium prepared from commercially available stem cell media supplements in an oxygenated rolling bottle culture system. Mouse embryos at E10.5 were carefully isolated from the uterus with intact yolk sac and in a process involving precise surgical maneuver the embryos were gently exteriorized from the yolk sac while maintaining the vascular continuity of the embryo with the yolk sac. Compared to embryos prepared with intact yolk sac or with the yolk sac removed, these embryos exhibited superior survival rate and developmental progression when cultured under similar conditions. We show that these mouse embryos, when cultured in a defined medium in an atmosphere of 95% O2 / 5% CO2 in a rolling bottle culture apparatus at 37 °C for 16-40 hr, exhibit morphological growth and development comparable to the embryos developing in utero. We believe this method will be useful for investigators needing to utilize whole embryo culture to study signaling interactions important in embryonic organogenesis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.