ArticleUltramicroscopy2014
Correlative and integrated light and electron microscopy of in-resin GFP fluorescence, used to localise diacylglycerol in mammalian cells.
Article in Ultramicroscopy, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
43 citing papers in PubMed, 128 citations in OpenAlex.
- Combining the CryoCapsule, HPM live µ and R221 conductive resin to track endosome from live imaging to electron microscopy using high-pressure freezing.Scientific reports · 2025Article
- The Inner Nuclear Membrane Has a Unique Lipid Signature.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Correlative light and electron microscopy imaging of proteinaceous deposits in cell cultures and brain tissues.Acta neuropathologica communications · 2025Article
- 5-ALA localises to the autophagy compartment and increases its fluorescence upon autophagy enhancement through caloric restriction and spermidine treatment in human glioblastoma.Biochemistry and biophysics reports · 2024Article
- The Highs and Lows of Memantine-An Autophagy and Mitophagy Inducing Agent That Protects Mitochondria.Cells · 2023Article
- Volume electron microscopy.Nature reviews. Methods primers · 2022Article
- One for All, All for One: A Close Look at In-Resin Fluorescence Protocols for CLEM.Frontiers in cell and developmental biology · 2022Review
- A versatile enhanced freeze-substitution protocol for volume electron microscopy.Frontiers in cell and developmental biology · 2022Article
- High-precision targeting workflow for volume electron microscopy.The Journal of cell biology · 2021Article
- A prion-like protein regulator of seed germination undergoes hydration-dependent phase separation.Cell · 2021Article
- A workflow for streamlined acquisition and correlation of serial regions of interest in array tomography.BMC biology · 2021Article
- Invadopodia Structure in 3D Environment Resolved by Near-Infrared Branding Protocol Combining Correlative Confocal and FIB-SEM Microscopy.International journal of molecular sciences · 2021Article
- Targeting Functionally Characterized Synaptic Architecture Using Inherent Fiducials and 3D Correlative Microscopy.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2021Article
- Enhancing Interrogation of Skeletal Muscle Samples for Informative Quantitative Data.Journal of neuromuscular diseases · 2021Review
- Preservation of Fluorescence Signal and Imaging Optimization for Integrated Light and Electron Microscopy.Frontiers in cell and developmental biology · 2021Article
- Integrated Array Tomography for 3D Correlative Light and Electron Microscopy.Frontiers in molecular biosciences · 2021Article
- Optimization of negative stage bias potential for faster imaging in large-scale electron microscopy.Journal of structural biology: X · 2021Article
- Pyrene Excimer-Based Fluorescent Labeling of Cysteines Brought into Close Proximity by Protein Dynamics: ASEM-Induced Thiol-Ene Click Reaction for High Spatial Resolution CLEM.International journal of molecular sciences · 2020Article
- High accuracy, fiducial marker-based image registration of correlative microscopy images.Scientific reports · 2019Article
- The Rényi divergence enables accurate and precise cluster analysis for localization microscopy.Bioinformatics (Oxford, England) · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 3 countries.
Funding
Abstract
Fluorescence microscopy of GFP-tagged proteins is a fundamental tool in cell biology, but without seeing the structure of the surrounding cellular space, functional information can be lost. Here we present a protocol that preserves GFP and mCherry fluorescence in mammalian cells embedded in resin with electron contrast to reveal cellular ultrastructure. Ultrathin in-resin fluorescence (IRF) sections were imaged simultaneously for fluorescence and electron signals in an integrated light and scanning electron microscope. We show, for the first time, that GFP is stable and active in resin sections in vacuo. We applied our protocol to study the subcellular localisation of diacylglycerol (DAG), a modulator of membrane morphology and membrane dynamics in nuclear envelope assembly. We show that DAG is localised to the nuclear envelope, nucleoplasmic reticulum and curved tips of the Golgi apparatus. With these developments, we demonstrate that integrated imaging is maturing into a powerful tool for accurate molecular localisation to structure.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.