ArticleBiosensors & bioelectronics2014
Evaluation of a biohybrid photoelectrochemical cell employing the purple bacterial reaction centre as a biosensor for herbicides.
Article in Biosensors & bioelectronics, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 84 citations in OpenAlex.
- Article
- Advances in biosensors for bacterial detection and identification.World journal of microbiology & biotechnology · 2025Review
- The Role of Electrostatic Binding Interfaces in the Performance of Bacterial Reaction Center Biophotoelectrodes.ACS sustainable chemistry & engineering · 2023Article
- Sustaining Electron Transfer Pathways Extends Biohybrid Photoelectrode Stability to Years.Angewandte Chemie (International ed. in English) · 2022Article
- Stabilisierung von Elektronentransferwegen erlaubt Stabilität von Biohybrid-Photoelektroden über Jahre.Angewandte Chemie (Weinheim an der Bergstrasse, Germany) · 2022Article
- Porous silicon pillar structures/photosynthetic reaction centre protein hybrid for bioelectronic applications.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2022Article
- Insight into Electron Transfer from a Redox Polymer to a Photoactive Protein.The journal of physical chemistry. B · 2020Article
- High-pressure tuning of primary photochemistry in bacterial photosynthesis: membrane-bound versus detergent-isolated reaction centers.Photosynthesis research · 2020Article
- Polychromatic solar energy conversion in pigment-protein chimeras that unite the two kingdoms of (bacterio)chlorophyll-based photosynthesis.Nature communications · 2020Article
- A Storable Mediatorless Electrochemical Biosensor for Herbicide Detection.Microorganisms · 2019Article
- Dynamics Properties of Photosynthetic Microorganisms Probed by Incoherent Neutron Scattering.Biophysical journal · 2019Article
- Both electronic and vibrational coherences are involved in primary electron transfer in bacterial reaction center.Nature communications · 2019Article
- Photosynthetic apparatus of Rhodobacter sphaeroides exhibits prolonged charge storage.Nature communications · 2019Article
- Modelling of the cathodic and anodic photocurrents from Rhodobacter sphaeroides reaction centres immobilized on titanium dioxide.Photosynthesis research · 2018Article
- Engineered photoproteins that give rise to photosynthetically-incompetent bacteria are effective as photovoltaic materials for biohybrid photoelectrochemical cells.Faraday discussions · 2018Article
- Spectrally selective fluorescence imaging of Chlorobaculum tepidum reaction centers conjugated to chelator-modified silver nanowires.Photosynthesis research · 2018Article
- Engineering of a calcium-ion binding site into the RC-LH1-PufX complex of Rhodobacter sphaeroides to enable ion-dependent spectral red-shifting.Biochimica et biophysica acta. Bioenergetics · 2017Article
- The effectiveness of styrene-maleic acid (SMA) copolymers for solubilisation of integral membrane proteins from SMA-accessible and SMA-resistant membranes.Biochimica et biophysica acta. Biomembranes · 2017Article
- Cytochrome c Provides an Electron-Funneling Antenna for Efficient Photocurrent Generation in a Reaction Center Biophotocathode.ACS applied materials & interfaces · 2017Article
- Sensing photosynthetic herbicides in an electrochemical flow cell.Photosynthesis research · 2017Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
The Rhodobacter sphaeroides reaction centre is a relatively robust and tractable membrane protein that has potential for exploitation in technological applications, including biohybrid devices for photovoltaics and biosensing. This report assessed the usefulness of the photocurrent generated by this reaction centre adhered to a small working electrode as the basis for a biosensor for classes of herbicides used extensively for the control of weeds in major agricultural crops. Photocurrent generation was inhibited in a concentration-dependent manner by the triazides atrazine and terbutryn, but not by nitrile or phenylurea herbicides. Measurements of the effects of these herbicides on the kinetics of charge recombination in photo-oxidised reaction centres in solution showed the same selectivity of response. Titrations of reaction centre photocurrents yielded half maximal inhibitory concentrations of 208nM and 2.1µM for terbutryn and atrazine, respectively, with limits of detection estimated at around 8nM and 50nM, respectively. Photocurrent attenuation provided a direct measure of herbicide concentration, with no need for model-dependent kinetic analysis of the signal used for detection or the use of prohibitively complex instrumentation, and prospects for the use of protein engineering to develop the sensitivity and selectivity of herbicide binding by the Rba. sphaeroides reaction centre are discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.