ArticleAnalytical biochemistry2014
Modification of gel architecture and TBE/TAE buffer composition to minimize heating during agarose gel electrophoresis.
Article in Analytical biochemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 47 citations in OpenAlex.
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- Spectrophotometric and nucleic acid-binding properties of halloysite clay nanotubes and kaolinite.Heliyon · 2023Article
- A Sensitive and Nonoptical CRISPR Detection Mechanism by Sizing Double-Stranded λ DNA Reporter.Angewandte Chemie (International ed. in English) · 2022Article
- Determination of Optimum Ratio of Cationic Polymers and Small Interfering RNA with Agarose Gel Retardation Assay.Methods in molecular biology (Clifton, N.J.) · 2022Article
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- pH-dependent sedimentation of DNA in the presence of divalent, but not monovalent, metal ions.Analytical biochemistry · 2021Article
- DifferentiationJournal of fungi (Basel, Switzerland) · 2021Article
- Genome-Wide DNA Methylation Analysis of Mammary Gland Tissues From Chinese Holstein Cows WithFrontiers in genetics · 2020Article
- High-Resolution Mapping of 3' Extremities of RNA Exosome Substrates by 3' RACE-Seq.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Horizontal Agarose Gel Mobility Shift Assay for Protein-RNA Complexes.Methods in molecular biology (Clifton, N.J.) · 2019Article
- Factors affecting the association of single- and double-stranded RNAs with montmorillonite nanoclays.International journal of biological macromolecules · 2018Article
- Rapid agarose gel electrophoretic mobility shift assay for quantitating protein: RNA interactions.Analytical biochemistry · 2016Article
- Identification of RNase-resistant RNAs in Saccharomyces cerevisiae extracts: Separation from chromosomal DNA by selective precipitation.Analytical biochemistry · 2016Article
- Impact of size, secondary structure, and counterions on the binding of small ribonucleic acids to layered double hydroxide nanoparticles.Biointerphases · 2015Article
- MethylRAD: a simple and scalable method for genome-wide DNA methylation profiling using methylation-dependent restriction enzymes.Open biology · 2015Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Agarose gel electrophoresis of DNA and RNA is routinely performed using buffers containing either Tris, acetate, and EDTA (TAE) or Tris, borate, and EDTA (TBE). Gels are run at a low, constant voltage (∼10 V/cm) to minimize current and asymmetric heating effects, which can induce band artifacts and poor resolution. In this study, alterations of gel structure and conductive media composition were analyzed to identify factors causing higher electrical currents during horizontal slab gel electrophoresis. Current was reduced when thinner gels and smaller chamber buffer volumes were used, but was not influenced by agarose concentration or the presence of ethidium bromide. Current was strongly dependent on the amount and type of EDTA used and on the concentrations of the major acid-base components of each buffer. Interestingly, resolution and the mobilities of circular versus linear plasmid DNAs were also affected by the chemical form and amount of EDTA. With appropriate modifications to gel structure and buffer constituents, electrophoresis could be performed at high voltages (20-25 V/cm), reducing run times by up to 3-fold. The most striking improvements were observed with small DNAs and RNAs (10-100 bp): high voltages and short run times produced sharper bands and higher resolution.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.