ArticleGenes & development2014
Impacts of the ubiquitous factor Zelda on Bicoid-dependent DNA binding and transcription in Drosophila.
Article in Genes & development, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 66 papers.
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66 citing papers in PubMed, 117 citations in OpenAlex.
- Asymmetric chromatin accessibility underlies anterior-posterior axis specification in moth fly embryos.PLoS biology · 2026Article
- Differential usage of two, distinct DNA-binding domains regulates tissue-specific occupancy of the pioneer factor Zelda.bioRxiv : the preprint server for biology · 2025Article
- Breaking anterior-posterior symmetry in the moth flybioRxiv : the preprint server for biology · 2025Article
- Bulk-level maps of pioneer factor binding dynamics during the Drosophila maternal-to-zygotic transition.Development (Cambridge, England) · 2025Article
- Catalytic-dependent and independent functions of the histone acetyltransferase CBP promote pioneer-factor-mediated zygotic genome activation.Molecular cell · 2025Article
- The maternal-to-zygotic transition: reprogramming of the cytoplasm and nucleus.Nature reviews. Genetics · 2025Review
- Transcription factor clusters as information transfer agents.Science advances · 2025Article
- Global coupling of R-loop dynamics with RNA polymerase II modulates gene expression and early development of Drosophila.Nucleic acids research · 2024Article
- Article
- Development and evolution of Drosophila chromatin landscape in a 3D genome context.Nature communications · 2024Article
- OVO positively regulates essential maternal pathways by binding near the transcriptional start sites in theeLife · 2024Article
- How Transcription Factor Clusters Shape the Transcriptional Landscape.Biomolecules · 2024Review
- OVO Positively Regulates Essential Maternal Pathways by Binding Near the Transcriptional Start Sites in thebioRxiv : the preprint server for biology · 2024Article
- Multi-level regulation of even-skipped stripes by the ubiquitous factor Zelda.Development (Cambridge, England) · 2023Article
- Chromatin accessibility in the Drosophila embryo is determined by transcription factor pioneering and enhancer activation.Developmental cell · 2023Article
- Review
- In Situ Hybridization as a Method to Examine Gene Regulatory Activity In Vivo.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Article
- Synthetic reconstruction of theeLife · 2022Article
- TheFaculty reviews · 2022Review
6 more citing papers are in PubMed but not listed here.
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6 authors at 1 institution in 1 country.
Funding
Abstract
In vivo cross-linking studies suggest that the Drosophila transcription factor Bicoid (Bcd) binds to several thousand sites during early embryogenesis, but it is not clear how many of these binding events are functionally important. In contrast, reporter gene studies have identified >60 Bcd-dependent enhancers, all of which contain clusters of the consensus binding sequence TAATCC. These studies also identified clusters of TAATCC motifs (inactive fragments) that failed to drive Bcd-dependent activation. In general, active fragments showed higher levels of Bcd binding in vivo and were enriched in predicted binding sites for the ubiquitous maternal protein Zelda (Zld). Here we tested the role of Zld in Bcd-mediated binding and transcription. Removal of Zld function and mutations in Zld sites caused significant reductions in Bcd binding to known enhancers and variable effects on the activation and spatial positioning of Bcd-dependent expression patterns. Also, insertion of Zld sites converted one of six inactive fragments into a Bcd-responsive enhancer. Genome-wide binding experiments in zld mutants showed variable effects on Bcd-binding peaks, ranging from strong reductions to significantly enhanced levels of binding. Increases in Bcd binding caused the precocious Bcd-dependent activation of genes that are normally not expressed in early embryos, suggesting that Zld controls the genome-wide binding profile of Bcd at the qualitative level and is critical for selecting target genes for activation in the early embryo. These results underscore the importance of combinatorial binding in enhancer function and provide data that will help predict regulatory activities based on DNA sequence.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.