ArticleMolecular and cellular biology2014
Ribosome assembly factors Pwp1 and Nop12 are important for folding of 5.8S rRNA during ribosome biogenesis in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 40 citations in OpenAlex.
- Periodic tryptophan protein 1 promotes colorectal cancer growth via ribosome biogenesis.International journal of clinical oncology · 2025Article
- PWP1 transcriptionally regulates p53, modulating apoptosis and cell cycle to promote gastric cancer progression.Apoptosis : an international journal on programmed cell death · 2025Article
- PWP1 is overexpressed in hepatocellular carcinoma and facilitates liver cancer cell proliferation.Heliyon · 2024Article
- Article
- Ribosome biogenesis factors-from names to functions.The EMBO journal · 2023Review
- Maize Shrek1 encodes a WD40 protein that regulates pre-rRNA processing in ribosome biogenesis.The Plant cell · 2022Article
- Emergence of the primordial pre-60S from the 90S pre-ribosome.Cell reports · 2022Article
- Puf6 primes 60S pre-ribosome nuclear export at low temperature.Nature communications · 2021Article
- Transcriptomic analysis of early stages of intestinal regeneration in Holothuria glaberrima.Scientific reports · 2021Article
- Bcl-2 Associated Athanogene 2 (BAG2) is Associated With Progression and Prognosis of Hepatocellular Carcinoma: A Bioinformatics-Based Analysis.Pathology oncology research : POR · 2021Article
- CRL4Science advances · 2020Article
- PWP1 Promotes the Malignant Phenotypes of Lung Cancer Cells by Interacting with DVL2 and Merlin.OncoTargets and therapy · 2020Article
- Rrp5 establishes a checkpoint for 60S assembly during 40S maturation.RNA (New York, N.Y.) · 2019Article
- Assembly and early maturation of large subunit precursors.RNA (New York, N.Y.) · 2019Article
- Enhancing co-translational folding of heterologous protein by deleting non-essential ribosomal proteins inBiotechnology for biofuels · 2019Article
- Ribosomal protein L14 contributes to the early assembly of 60S ribosomal subunits in Saccharomyces cerevisiae.Nucleic acids research · 2018Article
- Article
- High-throughput RNA structure probing reveals critical folding events during early 60S ribosome assembly in yeast.Nature communications · 2017Article
- Stepwise assembly of the earliest precursors of large ribosomal subunits in yeast.Nucleic acids research · 2017Article
- The assembly factor Erb1 functions in multiple remodeling events during 60S ribosomal subunit assembly in S. cerevisiae.Nucleic acids research · 2017Article
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5 authors at 1 institution in 1 country.
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Abstract
Previous work from our lab suggests that a group of interdependent assembly factors (A(3) factors) is necessary to create early, stable preribosomes. Many of these proteins bind at or near internal transcribed spacer 2 (ITS2), but in their absence, ITS1 is not removed from rRNA, suggesting long-range communication between these two spacers. By comparing the nonessential assembly factors Nop12 and Pwp1, we show that misfolding of rRNA is sufficient to perturb early steps of biogenesis, but it is the lack of A(3) factors that results in turnover of early preribosomes. Deletion of NOP12 significantly inhibits 27SA(3) pre-rRNA processing, even though the A(3) factors are present in preribosomes. Furthermore, pre-rRNAs are stable, indicating that the block in processing is not sufficient to trigger turnover. This is in contrast to the absence of Pwp1, in which the A(3) factors are not present and pre-rRNAs are unstable. In vivo RNA structure probing revealed that the pre-rRNA processing defects are due to misfolding of 5.8S rRNA. In the absence of Nop12 and Pwp1, rRNA helix 5 is not stably formed. Interestingly, the absence of Nop12 results in the formation of an alternative yet unproductive helix 5 when cells are grown at low temperatures.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.