ArticleClinical and translational medicine2014
Proper paraffin slide storage is crucial for translational research projects involving immunohistochemistry stains.
Article in Clinical and translational medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Spotlight on challenges and novel methods in highly multiplexed tissue imaging-based spatial proteomics.Journal of translational medicine · 2026Review
- Quantitative Protein Expression of Antibody-Drug Conjugate Targets in EGFR Mutated and Wild-type Non-Small Cell Lung Cancer.Clinical cancer research : an official journal of the American Association for Cancer Research · 2025Article
- FTO O-GlcNAcylation promotes TRIM21-mediated FTO ubiquitination degradation to sustain the negative feedback control of macrophage inflammation.Frontiers in immunology · 2025Article
- Classifying breast cancer and fibroadenoma tissue biopsies from paraffined stain-free slides by fractal biomarkers in Fourier Ptychographic Microscopy.Computational and structural biotechnology journal · 2024Article
- Multimodal and spatially resolved profiling identifies distinct patterns of T cell infiltration in nodal B cell lymphoma entities.Nature cell biology · 2024Article
- Effect of storage time of paraffin sections on the expression of PD-L1 (SP142) in invasive breast cancer.Diagnostic pathology · 2023Article
- Quality Assessment of Proteins and RNA Following Storage in Archival Formalin-Fixed Paraffin-Embedded Human Breast Cancer Tissue Microarray Sections.Biopreservation and biobanking · 2023Article
- Article
- Effects of Slide Storage on Detection of Molecular Markers by IHC and FISH in Endometrial Cancer Tissues From a Clinical Trial: An NRG Oncology/GOG Pilot Study.Applied immunohistochemistry & molecular morphology : AIMM · 2022Article
- Semi-automated background removal limits data loss and normalizes imaging mass cytometry data.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2021Article
- Pitfalls and Caveats in Applying Chromogenic Immunostaining to Histopathological Diagnosis.Cells · 2021Review
- Accelerated instability testing reveals quantitative mass spectrometry overcomes specimen storage limitations associated with PD-L1 immunohistochemistry.Laboratory investigation; a journal of technical methods and pathology · 2020Article
- Storage Conditions and Immunoreactivity of Breast Cancer Subtyping Markers in Tissue Microarray Sections.Applied immunohistochemistry & molecular morphology : AIMM · 2020Article
- Toward Improving Practices for Submission of Diagnostic Tissue Blocks for National Cancer Institute Clinical Trials.American journal of clinical pathology · 2020Article
- Enhancing the Value of Histopathological Assessment of Allograft Biopsy Monitoring.Transplantation · 2019Review
- Fresh Cut Versus Stored Cut Paraffin-embedded Tissue: Effect on Immunohistochemical Staining for Common Breast Cancer Markers.Applied immunohistochemistry & molecular morphology : AIMM · 2019Article
- In Situ Imaging of Tryptic Peptides by MALDI Imaging Mass Spectrometry Using Fresh-Frozen or Formalin-Fixed, Paraffin-Embedded Tissue.Current protocols in protein science · 2018Article
- The development of a Biobank of cancer tissue samples from World Trade Center responders.Journal of translational medicine · 2018Article
- Principles and approaches for reproducible scoring of tissue stains in research.Laboratory investigation; a journal of technical methods and pathology · 2018Review
- Section detachment in immunohistochemistry: causes, troubleshooting, and problem-solving.Histochemistry and cell biology · 2017Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The use of paraffin slides and tissue microarrays (TMA) is indispensable for translational research. However, storage of paraffin slides over time has a substantial detrimental effect on the quality and reliability of immunohistochemistry stains. Particularly affected by this issue may be any collaborative efforts where paraffin slides or TMAs are shipped to central laboratories and then 'biobanked' for some time until use. This article summarizes some of the key issues affecting loss of antigenicity on paraffin slides and some simple storage solutions to help maintain high quality immunohistochemistry results when paraffin slides must be stored for a certain time prior to use.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.