ArticleBreast cancer research : BCR2014
Next-generation transcriptome sequencing of the premenopausal breast epithelium using specimens from a normal human breast tissue bank.
Article in Breast cancer research : BCR, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- Transcriptomic Profile of Breast Tissue of Premenopausal Women Following Treatment with Progesterone Receptor Modulator: Secondary Outcomes of a Randomized Controlled Trial.International journal of molecular sciences · 2024Trial
- Single cell RNA-sequencing reveals that testosterone reduces estrogen signaling in the healthy human mammary gland.Journal of mammary gland biology and neoplasia · 2026Article
- Single cell RNA-sequencing reveals an association between testosterone treatment and reduced hormone signaling in the human mammary gland.bioRxiv : the preprint server for biology · 2026Article
- Conception and Concern: A Review of Breast Cancer Risk in Assisted Reproductive Technology.International journal of biological sciences · 2025Review
- Hormone Signaling in Breast Development and Cancer.Advances in experimental medicine and biology · 2025Review
- Host, reproductive, and lifestyle factors in relation to quantitative histologic metrics of the normal breast.Breast cancer research : BCR · 2023Article
- Progesterone from ovulatory menstrual cycles is an important cause of breast cancer.Breast cancer research : BCR · 2023Review
- ERBB Receptors and Their Ligands in the Developing Mammary Glands of Different Species: Fifteen Characters in Search of an Author.Journal of mammary gland biology and neoplasia · 2023Review
- What Is Known about Breast Cancer in Young Women?Cancers · 2023Review
- Overexpression of Estrogen Receptor α in Mammary Glands of Aging Mice Is Associated with a Proliferative Risk Signature and Generation of Estrogen Receptor α-Positive Mammary Adenocarcinomas.The American journal of pathology · 2023Article
- Oncobiology and treatment of breast cancer in young women.Cancer metastasis reviews · 2022Review
- Mapping hormone-regulated cell-cell interaction networks in the human breast at single-cell resolution.Cell systems · 2022Article
- Article
- Characterization of transcriptome diversity and in vitro behavior of primary human high-risk breast cells.Scientific reports · 2022Article
- Article
- Progesterone receptor antagonists reverse stem cell expansion and the paracrine effectors of progesterone action in the mouse mammary gland.Breast cancer research : BCR · 2021Article
- RSK2 and ERα comrades-in-arms in homeostasis and transformation.Molecular & cellular oncology · 2020Article
- RSK2 Maintains Adult Estrogen Homeostasis by Inhibiting ERK1/2-Mediated Degradation of Estrogen Receptor Alpha.Cell reports · 2020Article
- Progesterone and Breast Cancer.Endocrine reviews · 2020Review
- Upregulation of lipid metabolism genes in the breast prior to cancer diagnosis.NPJ breast cancer · 2020Article
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21 authors.
Funding
Abstract
introductionOur efforts to prevent and treat breast cancer are significantly impeded by a lack of knowledge of the biology and developmental genetics of the normal mammary gland. In order to provide the specimens that will facilitate such an understanding, The Susan G. Komen for the Cure Tissue Bank at the IU Simon Cancer Center (KTB) was established. The KTB is, to our knowledge, the only biorepository in the world prospectively established to collect normal, healthy breast tissue from volunteer donors. As a first initiative toward a molecular understanding of the biology and developmental genetics of the normal mammary gland, the effect of the menstrual cycle and hormonal contraceptives on DNA expression in the normal breast epithelium was examined.
methodsUsing normal breast tissue from 20 premenopausal donors to KTB, the changes in the mRNA of the normal breast epithelium as a function of phase of the menstrual cycle and hormonal contraception were assayed using next-generation whole transcriptome sequencing (RNA-Seq).
resultsIn total, 255 genes representing 1.4% of all genes were deemed to have statistically significant differential expression between the two phases of the menstrual cycle. The overwhelming majority (221; 87%) of the genes have higher expression during the luteal phase. These data provide important insights into the processes occurring during each phase of the menstrual cycle. There was only a single gene significantly differentially expressed when comparing the epithelium of women using hormonal contraception to those in the luteal phase.
conclusionsWe have taken advantage of a unique research resource, the KTB, to complete the first-ever next-generation transcriptome sequencing of the epithelial compartment of 20 normal human breast specimens. This work has produced a comprehensive catalog of the differences in the expression of protein-coding genes as a function of the phase of the menstrual cycle. These data constitute the beginning of a reference data set of the normal mammary gland, which can be consulted for comparison with data developed from malignant specimens, or to mine the effects of the hormonal flux that occurs during the menstrual cycle.
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