ArticleFrontiers in cellular and infection microbiology2014
Brucella dissociation is essential for macrophage egress and bacterial dissemination.
Article in Frontiers in cellular and infection microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
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- Intracellular growth of Brucella is mediated by Dps-dependent activation of ferritinophagy.EMBO reports · 2023Article
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- Protective Effects againstJournal of microbiology and biotechnology · 2023Article
- The mechanism of chronic intracellular infection withFrontiers in cellular and infection microbiology · 2023Review
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- Intracellular Passage Triggers a Molecular Response in Brucella abortus That Increases Its Infectiousness.Infection and immunity · 2021Article
- Protection of palmitic acid treatment in RAW264.7 cells and BALB/c mice duringJournal of veterinary science · 2021Article
- Lethality ofVirulence · 2019Article
- Doxycycline-encapsulated solid lipid nanoparticles for the enhanced antibacterial potential to treat the chronic brucellosis and preventing its relapse: in vivo study.Annals of clinical microbiology and antimicrobials · 2019Article
- Harnessing Macrophages for Controlled-Release Drug Delivery: Lessons From Microbes.Frontiers in pharmacology · 2019Review
- Guanylate-binding protein 5 licenses caspase-11 for Gasdermin-D mediated host resistance to Brucella abortus infection.PLoS pathogens · 2018Article
- The Key Role of c-Fos for Immune Regulation and Bacterial Dissemination inFrontiers in cellular and infection microbiology · 2018Article
- Characterization of Brucella abortus mutant strain Δ22915, a potential vaccine candidate.Veterinary research · 2017Article
- Contrasting Lifestyles Within the Host Cell.Microbiology spectrum · 2016Review
- Establishment of Chronic Infection: Brucella's Stealth Strategy.Frontiers in cellular and infection microbiology · 2016Review
- Brucella suis Vaccine Strain 2 Induces Endoplasmic Reticulum Stress that Affects Intracellular Replication in Goat Trophoblast Cells In vitro.Frontiers in cellular and infection microbiology · 2016Article
- Adrenal steroids modulate the immune response during Brucella abortus infection by a mechanism that depends on the regulation of cytokine production.Infection and immunity · 2015Article
- Smooth to Rough Dissociation in Brucella: The Missing Link to Virulence.Frontiers in cellular and infection microbiology · 2015Article
- Microbial modulation of host apoptosis and pyroptosis.Frontiers in cellular and infection microbiology · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
It has long been observed that smooth Brucella can dissociate into rough mutants that are cytotoxic to macrophages. However, the in vivo biological significance and/or mechanistic details of Brucella dissociation and cytotoxicity remain incomplete. In the current report, a plaque assay was developed using Brucella strains exhibiting varying degrees of cytotoxicity. Infected monolayers were observed daily using phase contrast microscopy for plaque formation while Brucella uptake and replication were monitored using an immunofluorescence assay (IFA). Visible plaques were detected at 4-5 days post infection (p.i.) with cytotoxic Brucella 16MΔmanBA at an MOI of 0.1. IFA staining demonstrated that the plaques consisted of macrophages with replicating Brucella. Visible plaques were not detected in monolayers infected with non-cytotoxic 16MΔmanBAΔvirB2 at an MOI of 0.1. However, IFA staining did reveal small groups of macrophages (foci) with replicating Brucella in the monolayers infected with 16MΔmanBAΔvirB2. The size of the foci observed in macrophage monolayers infected with rough Brucella correlated directly with cytotoxicity measured in liquid culture, suggesting that cytotoxicity was essential for Brucella egress and dissemination. In monolayers infected with 16M, small and large foci were observed. Double antibody staining revealed spontaneous rough mutants within the large, but not the small foci in 16M infected monolayers. Furthermore, plaque formation was observed in the large foci derived from 16M infections. Finally, the addition of gentamicin to the culture medium inhibited plaque formation, suggesting that cell-to-cell spread occurred only following release of the organisms from the cells. Taken together, these results demonstrate that Brucella-induced cytotoxicity is critical for Brucella egress and dissemination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.