Evidence map›Paper›PMID 24634834›Full record

ArticleRedox biology2014

Reductive stress after exercise: The issue of redox individuality.

N V Margaritelis, A Kyparos, V Paschalis, A A Theodorou, G Panayiotou, A Zafeiridis, K Dipla, M G Nikolaidis, I S Vrabas

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Redox biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
40citing papers in PubMed, 1 pooled it
9.3field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

40 citing papers in PubMed, 1 synthesis or guideline pooled it, 86 citations in OpenAlex.

  1. Pooled it
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  7. Article
  8. Article
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  10. Nanomedicine-Driven Modulation of Reductive Stress for Cancer Therapy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Review
  11. Article
  12. Article
  13. Ten "Cheat Codes" for Measuring Oxidative Stress in Humans.Antioxidants (Basel, Switzerland) · 2024
    Review
  14. Review
  15. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

N V MargaritelisExercise Physiology and Biochemistry Laboratory, Department of Physical Education and Sports Science at Serres, Aristotle University of Thessaloniki, Agios Ioannis, Serres 62110, Greece.
A KyparosExercise Physiology and Biochemistry Laboratory, Department of Physical Education and Sports Science at Serres, Aristotle University of Thessaloniki, Agios Ioannis, Serres 62110, Greece.
V PaschalisDepartment of Physical Education and Sports Science, University of Thessaly, Karies, Trikala 42100, Greece ; Laboratory of Exercise, Health and Human Performance, Research Center, European University of Cyprus, Nicosia, Cyprus.
A A TheodorouExercise Physiology and Biochemistry Laboratory, Department of Physical Education and Sports Science at Serres, Aristotle University of Thessaloniki, Agios Ioannis, Serres 62110, Greece ; Laboratory of Exercise, Health and Human Performance, Research Center, European University of Cyprus, Nicosia, Cyprus.
G PanayiotouLaboratory of Exercise, Health and Human Performance, Research Center, European University of Cyprus, Nicosia, Cyprus.
A ZafeiridisExercise Physiology and Biochemistry Laboratory, Department of Physical Education and Sports Science at Serres, Aristotle University of Thessaloniki, Agios Ioannis, Serres 62110, Greece.
K DiplaExercise Physiology and Biochemistry Laboratory, Department of Physical Education and Sports Science at Serres, Aristotle University of Thessaloniki, Agios Ioannis, Serres 62110, Greece.
M G NikolaidisExercise Physiology and Biochemistry Laboratory, Department of Physical Education and Sports Science at Serres, Aristotle University of Thessaloniki, Agios Ioannis, Serres 62110, Greece.
I S VrabasExercise Physiology and Biochemistry Laboratory, Department of Physical Education and Sports Science at Serres, Aristotle University of Thessaloniki, Agios Ioannis, Serres 62110, Greece.
Aristotle University of Thessaloniki · GREuropean University Cyprus · CYUniversity of Cyprus · CY

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Exercise has been consistently used as an oxidant stimulus in redox biology studies. However, previous studies have focused on group differences and did not examine individual differences. As a result, it remains untested whether all individuals experience oxidative stress after acute exercise. Therefore, the main aim of the present study was to investigate whether some individuals exhibit unexpected responses after an acute eccentric (i.e., muscle-damaging) exercise session. Ninety eight (N = 98) young men performed an isokinetic eccentric exercise bout with the knee extensors. Plasma, erythrocytes and urine samples were collected immediately before and 2 days post-exercise. Three commonly used redox biomarkers (F2-isoprostanes, protein carbonyls and glutathione) were assayed. As expected, the two oxidant biomarkers (F2-isoprostanes and protein carbonyls) significantly increased 2 days after exercise (46% and 61%, respectively); whereas a significant decrease in glutathione levels (by -21%) was observed after exercise. A considerable number of the participants exhibited changes in the levels of biomarkers in the opposite, unexpected direction than the group average. More specifically, 13% of the participants exhibited a decrease in F2-isoprostanes and protein carbonyls and 10% of the participants exhibited an increase in glutathione levels. Furthermore, more than 1 out of 3 individuals exhibited either unexpected or negligible (from 0% to ± 5%) responses to exercise in at least one redox biomarker. It was also observed that the initial values of redox biomarkers are important predictors of the responses to exercise. In conclusion, although exercise induces oxidative stress in the majority of individuals, it can induce reductive stress or negligible stress in a considerable number of people. The data presented herein emphasize that the mean response to a redox stimulus can be very misleading. We believe that the wide variability (including the cases of reductive stress) described is not limited to the oxidant stimulus used and the biomarkers selected.

Indexed as

Oxidative StressAdultBiomarkersExercise TestF2-IsoprostanesGlutathioneHumansMaleMuscle, SkeletalProtein CarbonylationReactive Oxygen SpeciesYoung AdultBiomarkersF2-IsoprostanesGlutathioneReactive Oxygen SpeciesAntioxidantsFree radicalsIndividualityMuscle damageOxidative stressRedox biology

Identifiers

PMID24634834
PMCPMC3953955
OpenAlexW2069997942

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.