ArticleThe Journal of biological chemistry2014
Uncoupling of ionic currents from substrate transport in the plant ammonium transporter AtAMT1;2.
Article in The Journal of biological chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Sustainable stress management in crops: unlocking the potential of rhizospheric microbes.Archives of microbiology · 2025Review
- Plant-microbe interactions in the rhizosphere for smarter and more sustainable crop fertilization: the case of PGPR-based biofertilizers.Frontiers in microbiology · 2024Review
- Identification and functional characterization of the sugarcane (Frontiers in plant science · 2023Article
- Regulation of Cytosolic pH: The Contributions of Plant Plasma Membrane HInternational journal of molecular sciences · 2021Review
- Nitrogen Uptake in Plants: The Plasma Membrane Root Transport Systems from a Physiological and Proteomic Perspective.Plants (Basel, Switzerland) · 2021Review
- Ammonium transporter expression in sperm of the disease vectorProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Function and Regulation of Ammonium Transporters in Plants.International journal of molecular sciences · 2020Review
- Functional Characterization of theFrontiers in plant science · 2020Article
- Ammonium transceptors: Novel regulators of fungal development.PLoS pathogens · 2019Article
- Ammonia Excretion in an Osmoregulatory Syncytium Is Facilitated by AeAmt2, a Novel Ammonia Transporter inFrontiers in physiology · 2018Article
- Structural basis for Mep2 ammonium transceptor activation by phosphorylation.Nature communications · 2016Article
- Evolution of Electrogenic Ammonium Transporters (AMTs).Frontiers in plant science · 2016Article
- Antennal-expressed ammonium transporters in the malaria vector mosquito Anopheles gambiae.PloS one · 2014Article
- Switching substrate specificity of AMT/MEP/ Rh proteins.Channels (Austin, Tex.) · 2014Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ammonium flux across prokaryotic, plant, and animal membranes is regulated by structurally related ammonium transporters (AMT) and/or related Rhesus (Rh) glycoproteins. Several plant AMT homologs, such as AtAMT1;2 from Arabidopsis, elicit ionic, ammonium-dependent currents when expressed in oocytes. By contrast, functional evidence for the transport of NH3 and the lack of coupled ionic currents has been provided for many Rh proteins. Furthermore, despite high resolution structures the transported substrate in many bacterial homologs, such as AmtB from Escherichia coli, is still unclear. In a heterologous genetic screen in yeast, AtAMT1;2 mutants with reduced transport activity were identified based on the resistance of yeast to the toxic transport analog methylamine. When expressed in oocytes, the reduced transport capacity was confirmed for either of the mutants Q67K, M72I,and W145S. Structural alignments suggest that these mutations were dispersed at subunit contact sites of trimeric AMTs, without direct contact to the pore lumen. Surprisingly, and in contrast to the wild type AtAMT1;2 transporter, ionic currents were not associated with the substrate transport in these mutants. Whether these data suggest that the wild type AtAMT1;2 functions as H(+)/NH3 co-transporter, as well as how the strict substrate coupling with protons is lost by the mutations, is discussed.
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