ArticleJournal of bacteriology2014
Biochemical characterization of a nitrogen-type phosphotransferase system reveals that enzyme EI(Ntr) integrates carbon and nitrogen signaling in Sinorhizobium meliloti.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 41 citations in OpenAlex.
- Bacterial nitrogen-related phosphotransferase systems: new insights into diverse roles in virulence, physiology, and central metabolism.Journal of bacteriology · 2026Review
- Regulation of proteolysis of the sigma factor RpoS by the Gac-Rsm signal transduction system inMicrobiology (Reading, England) · 2026Article
- Cell cycle control and environmental response by second messengers in Caulobacter crescentus.BMC bioinformatics · 2020Article
- Global control of bacterial nitrogen and carbon metabolism by a PTSProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Make and break the alarmone: regulation of (p)ppGpp synthetase/hydrolase enzymes in bacteria.FEMS microbiology reviews · 2019Review
- Determination of protein phosphorylation by polyacrylamide gel electrophoresis.Journal of microbiology (Seoul, Korea) · 2019Article
- Regulation of (p)ppGpp hydrolysis by a conserved archetypal regulatory domain.Nucleic acids research · 2019Article
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- GigA and GigB are Master Regulators of Antibiotic Resistance, Stress Responses, and Virulence in Acinetobacter baumannii.Journal of bacteriology · 2017Article
- Hit the right spots: cell cycle control by phosphorylated guanosines in alphaproteobacteria.Nature reviews. Microbiology · 2017Review
- Loss of malic enzymes leads to metabolic imbalance and altered levels of trehalose and putrescine in the bacterium Sinorhizobium meliloti.BMC microbiology · 2016Article
- Phosphotransferase-dependent accumulation of (p)ppGpp in response to glutamine deprivation in Caulobacter crescentus.Nature communications · 2016Article
- Genetic Analysis Reveals the Essential Role of Nitrogen Phosphotransferase System Components in Sinorhizobium fredii CCBAU 45436 Symbioses with Soybean and Pigeonpea Plants.Applied and environmental microbiology · 2016Article
- The Emergence of 2-Oxoglutarate as a Master Regulator Metabolite.Microbiology and molecular biology reviews : MMBR · 2015Review
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- Article
- Dephosphorylated NPr is involved in an envelope stress response of Escherichia coli.Microbiology (Reading, England) · 2015Article
- Site-Specific Ser/Thr/Tyr Phosphoproteome of Sinorhizobium meliloti at Stationary Phase.PloS one · 2015Article
- The bacterial phosphoenolpyruvate:carbohydrate phosphotransferase system: regulation by protein phosphorylation and phosphorylation-dependent protein-protein interactions.Microbiology and molecular biology reviews : MMBR · 2014Review
- The phosphohistidine phosphatase SixA dephosphorylates the phosphocarrier NPr.The Journal of biological chemistryArticle
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In Sinorhizobium meliloti, catabolite repression is influenced by a noncanonical nitrogen-type phosphotransferase system (PTS(Ntr)). In this PTS(Ntr), the protein HPr is phosphorylated on histidine-22 by the enzyme EI(Ntr) and the flux of phosphate through this residue onto downstream proteins leads to an increase in succinate-mediated catabolite repression (SMCR). In order to explore the molecular determinants of HPr phosphorylation by EI(Ntr), both proteins were purified and the activity of EI(Ntr) was measured. Experimentally determined kinetic parameters of EI(Ntr) activity were significantly slower than those determined for the carbohydrate-type EI in Escherichia coli. Enzymatic assays showed that glutamine, a signal of nitrogen availability in many Gram-negative bacteria, strongly inhibits EI(Ntr). Binding experiments using the isolated GAF domain of EI(Ntr) (EIGAF) showed that it is the domain responsible for detection of glutamine. EI(Ntr) activity was not affected by α-ketoglutarate, and no binding between the EIGAF and α-ketoglutarate could be detected. These data suggest that in S. melilloti, EI(Ntr) phosphorylation of HPr is regulated by signals from both carbon metabolism (phosphoenolpyruvate) and nitrogen metabolism (glutamine).
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